Polymorphism in the ITS region of ribosomal DNA of Cochliobolus sativus isolates differing in xylanase production

The restriction of PCR-amplified internal transcribed spacers (ITS) ofribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular...

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Bibliographic Details
Published in:Polish journal of microbiology 2008, Vol.57 (1), p.77-79
Main Authors: Bakri, Yasser, Jawhar, Mohammed, Arabi, Mohammed Imad Eddin
Format: Article
Language:English
Subjects:
Ascomycota - classification
Ascomycota - enzymology
Ascomycota - genetics
Ascomycota - isolation & purification
Cochliobolus sativus
Deoxyribonucleic acid
DNA
DNA, Fungal - analysis
DNA, Ribosomal Spacer - genetics
Endo-1,4-beta Xylanases - biosynthesis
Genetic Variation
Mycological Typing Techniques
Polymerase Chain Reaction
Polymorphism
Polymorphism, Restriction Fragment Length
Syria
Xylanase
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Summary:The restriction of PCR-amplified internal transcribed spacers (ITS) ofribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular parameter used showed that C. sativus isolates reside in three phylogenetic groups. There was observed the resolution between clustering of isolates and their xylanase production level.
ISSN:1733-1331
2544-4646