Receptor tyrosine kinase tie 1 mRNA is upregulated on cerebral microvessels after embolic middle cerebral artery occlusion in rat

• Published in: Brain research 1999-11, Vol.847 (2), p.338-342
• Main Authors: Zhang, Zheng Gang, Chopp, Michael, Lu, Dunyue, Wayne, Tsang, Zhang, Rui Lan, Morris, Daniel
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cerebral microvessel; Embolic MCA occlusion; In situ hybridization; Infarction, Middle Cerebral Artery - metabolism; Male; Medical sciences; Microcirculation; Neovascularization, Physiologic - physiology; Neurology; Rat; Rats; Rats, Wistar; Receptor Protein-Tyrosine Kinases - metabolism; Receptor, TIE-1; Receptors, Cell Surface - metabolism; Receptors, TIE; RNA, Messenger - metabolism; Tie 1; Vascular diseases and vascular malformations of the nervous system
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(99)02013-2

Tie 1 is an endothelial specific transmembrane receptor tyrosine kinase and may be required during angiogenesis. Using in situ hybridization, we measured tie 1 mRNA in ischemic brain ( n=15). Rats were subjected to middle cerebral artery (MCA) occlusion by a single fibrin rich clot. Expression of tie 1 was not detected in non ischemic brain. Cerebral microvessels expressed tie 1 in the ischemic lesion as early as 2 h after MCA occlusion. The number of microvessels containing tie 1 mRNA decreased in the ischemic lesion at 8 h after MCA occlusion. However, expression of tie 1 increased on microvessels at 24 h and 14 days after ischemia and tie 1 was primarily localized to the microvessels bordering pan necrotic tissue. Ninety-seven percent of cerebral vessels which expressed tie 1 mRNA had diameters of 3.7±0.17 μm. Our findings suggest a role for tie 1 in cerebral microvascular remodeling after embolic stroke.