Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene

Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, the DEV UL5 gene was cloned and sequenced from a vaccine virus. According to the consensus sequence of herpesvirus UL5 and UL3 gene degenerate oligonucleotide primers were designe...

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Bibliographic Details
Published in:Virus research 2008-09, Vol.136 (1), p.152-156
Main Authors: Pan, Huaqi, Cao, Ruibing, Liu, Lei, Niu, Mingfu, Zhou, Bin, Chen, Puyan, Hu, Jiangchun
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cloning, Molecular
Cluster Analysis
DNA Primers - genetics
DNA, Viral - chemistry
DNA, Viral - genetics
Duck enteritis virus
Ducks - virology
Gallid herpesvirus 2
Gallid herpesvirus 3
Gene character
Genotype
Herpesviridae - classification
Herpesviridae - genetics
Herpesvirus
Meleagrid herpesvirus 1
Molecular Sequence Data
Open Reading Frames
Phylogenetic analysis
Phylogeny
Polymerase Chain Reaction - methods
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
UL5 gene
Viral Proteins - genetics
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Summary:Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, the DEV UL5 gene was cloned and sequenced from a vaccine virus. According to the consensus sequence of herpesvirus UL5 and UL3 gene degenerate oligonucleotide primers were designed and were used in the polymerase chain reaction (PCR) to amplify DNA products with 4577 bp in size. DNA sequence analysis revealed a 2568 bp open reading frame (ORF) encoding a 855 amino acid polypeptide homologous to herpesvirus UL5 proteins. The DEV UL5 gene has a base composition of 769 adenine (29.95%), 556 cytosine (21.65%), 533 guanine (20.76%) and 710 thymine (27.65%). Sequence comparison revealed that the nucleotide sequence of the DEV UL5 gene was highly similar to other alphaherpesviruses. Phylogenetic tree analysis showed that the fifteen herpesviruses viruses analyzed fell into four large groups, and the duck enteritis virus itself branched and was most closely related to meleagrid herpesvirus 1, gallid herpesvirus 2 and gallid herpesvirus 3 subtrees.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2008.05.003