Expression of the Anti-apoptotic Factors Bcl-2 and Survivin in Canine Vascular Tumours

To investigate whether anti-apoptotic factors play a role in the malignant growth of canine haemangiosarcomas (HSAs), 83 HSAs and 22 haemangiomas were examined immunohistochemically for bcl-2 and survivin expression. Additionally, bcl-2 and survivin mRNA expression was quantified by semiquantitative...

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Bibliographic Details
Published in:Journal of comparative pathology 2008-07, Vol.139 (1), p.1-7
Main Authors: Murakami, M., Sakai, H., Kodama, A., Mori, T., Maruo, K., Yanai, T., Masegi, T.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
bcl-2
Cell Proliferation
dog
Dog Diseases - metabolism
Dog Diseases - pathology
Dogs
gene expression
Gene Expression Regulation, Neoplastic
haemangioma
haemangiosarcoma
hemangioma
Hemangioma - metabolism
Hemangioma - pathology
Hemangioma - veterinary
hemangiosarcoma
Hemangiosarcoma - metabolism
Hemangiosarcoma - pathology
Hemangiosarcoma - veterinary
immunohistochemistry
malignancy
messenger RNA
Neoplasm Proteins - metabolism
protein synthesis
Proto-Oncogene Proteins c-bcl-2 - metabolism
regulatory proteins
RNA, Messenger - metabolism
survivin
tumour
Vascular Neoplasms - metabolism
Vascular Neoplasms - pathology
Vascular Neoplasms - veterinary
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Summary:To investigate whether anti-apoptotic factors play a role in the malignant growth of canine haemangiosarcomas (HSAs), 83 HSAs and 22 haemangiomas were examined immunohistochemically for bcl-2 and survivin expression. Additionally, bcl-2 and survivin mRNA expression was quantified by semiquantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). Immunolabelling for bcl-2 was observed in 50 of the 83 HSA samples (60.2%) but in none of the haemangiomas. The average survivin positive index was 24.7% in the HSAs and 0.6% in the haemangiomas. In contrast to the high average value for survivin mRNA expression, which was approximately six times that for the haemangiomas, no significant difference was observed between HSAs and haemangiomas for the average bcl-2 mRNA expression level. The discrepancy between bcl-2 mRNA and bcl-2 protein expression requires further investigation, but the results suggest that malignant proliferation in canine HSAs is associated with bcl-2 and survivin expression.
ISSN:0021-9975
1532-3129
DOI:10.1016/j.jcpa.2008.02.001