Structural study of fucoidan from Cladosiphon okamuranus TOKIDA

A structural study was carried out on a fucoidan isolated from the brown seaweed Cladosiphon okamuranus. The polysaccharide contained fucose, glucuronic acid and sulfate in a molar ratio of about 6.1 : 1.0 : 2.9. The results of Smith degradation showed that this polysaccharide has a linear backbone...

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Bibliographic Details
Published in:Glycoconjugate journal 1999-01, Vol.16 (1), p.19-26
Main Authors: Nagaoka, M, Shibata, H, Kimura-Takagi, I, Hashimoto, S, Kimura, K, Makino, T, Aiyama, R, Ueyama, S, Yokokura, T
Format: Article
Language:English
Subjects:
Algae
Molecular biology
Molecular Structure
Polysaccharides - chemistry
Seaweed - chemistry
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Summary:A structural study was carried out on a fucoidan isolated from the brown seaweed Cladosiphon okamuranus. The polysaccharide contained fucose, glucuronic acid and sulfate in a molar ratio of about 6.1 : 1.0 : 2.9. The results of Smith degradation showed that this polysaccharide has a linear backbone of 1-->3-linked alpha-fucopyranose with a half sulfate substitution at the 4-positions, and a portion of the fucose residues was O-acetylated. The data obtained from partial acid hydrolysis, a methylation analysis and NMR spectra indicated that the alpha-glucuronic acid residue is linked to the 2-positions of the fucose residues, which were not substituted by a sulfate group. These results indicated that the average structure of this fucoidan is as follows: -[(-->3Fuc-4(+/-OSO3-)alpha1-)5-->3[GlcA alpha1-->2]Fuc alpha1-]n-. (Half of each fucose residue was sulfated. One O-acetyl ester was present in every 6 fucose residues.)
ISSN:0282-0080
1573-4986
DOI:10.1023/a:1006945618657