Fungistatic activity of iron-free bovin lactoferrin against several fungal plant pathogens and antagonists

• Published in: Natural product research 2008-07, Vol.22 (11), p.955-961
• Main Authors: Lahoz, Ernesto, Pisacane, Anna, Iannaccone, Marco, Palumbo, Daniela, Capparelli, Rosanna
• Format: Article
• Language: English
• Subjects: Alternaria - drug effects; Alternaria alternata; Animals; Ascomycota - drug effects; Aspergillus niger; Aspergillus niger - drug effects; Bovine lactoferrin; Candida albicans; Candida albicans - drug effects; Cattle; Colletotrichum - drug effects; Colletotrichum lindemuthianum; Fungi - drug effects; Fungistasis; Fusarium - drug effects; Fusarium solani; Gliocladium - drug effects; Gliocladium roseum; IC50 values; Iron - chemistry; Lactoferrin - chemistry; Lactoferrin - pharmacology; Microbial Sensitivity Tests; Phoma exigua; Plant Diseases - microbiology; Plants - microbiology; Rhizoctonia - drug effects; Rhizoctonia solani; Sclerotinia sclerotiorum; Sclerotium rolfsii; Soil-borne pathogens, antagonists; Trichoderma - drug effects; Trichoderma viride
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786410701650253

Lactoferrin (LF) is a member of the transferrin family of iron-binding glycoproteins. It is also a multifunctional protein of 80 kDa that is synthesized by glandular epithelial cells and secreted into mucosal fluid. High levels of LF are present in colostrom and milk and low levels in tears, saliva, and gastrointestinal and reproductive secretions. Data regarding the antifungal effects of LF are limited. Studies have been performed on Candida albicans, which demonstrated that LF inhibits the growth of this fungus. This study reports the results of experiments carried out in order to evaluate the effects of LF on the growth of 11 fungi, which were isolated from plants and soils. These experiments employed the methods of amended agar utilizing nine different concentration levels of LF (0, 0.001, 0.01, 0.1, 1, 10, 100, 1000, 5000 mg L −1 ). The effects of LF on the growth of these fungi were based on measures of the radial growth of the fungal colonies expressed both as percentage of inhibition and as IC 50 values (the concentration at which the fungal growth was inhibited by 50% relative to controls). LF had no effects on Alternaria alternata, Gliocladium roseum, Fusarium solani and Colletotrichum lindemuthianum. It did, however, inhibit the growth of Aspergillus niger, Trichoderma viride, Sclerotinia sclerotiorum, Sclerotium rolfsii, Rhizoctonia solani and Phoma exigua to the point that their IC 50 values ranged from 31.1 mg L −1 for S. sclerotiorum to 952 mg L −1 for T. viride.