Facts and artifacts in proteomics of body fluids. What proteomics of saliva is telling us

This review briefly depicts several salient points of the current status of knowledge on salivary peptidoma. It outlines the intrinsic difficulties in its characterization connected to different factors of variability, such as: i) the high genetic polymorphisms, complicated by individual insertions/...

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Bibliographic Details
Published in:Journal of separation science 2008-06, Vol.31 (11), p.1948-1963
Main Authors: Messana, Irene, Inzitari, Rosanna, Fanali, Chiara, Cabras, Tiziana, Castagnola, Massimo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Human
Humans
Molecular Sequence Data
Peptide
Protein
Proteins
Proteome - chemistry
Proteome - genetics
Proteome - metabolism
Proteomics
Saliva
Saliva - chemistry
Saliva - metabolism
Salivary Proteins and Peptides - chemistry
Salivary Proteins and Peptides - genetics
Salivary Proteins and Peptides - metabolism
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Summary:This review briefly depicts several salient points of the current status of knowledge on salivary peptidoma. It outlines the intrinsic difficulties in its characterization connected to different factors of variability, such as: i) the high genetic polymorphisms, complicated by individual insertions/deletions and alternative splicing; ii) complex post-translational maturations comprehending different proteolytic cleavages, glycosylation, phosphorylation and sulfation processes; iii) physiological variations and different contributions to the whole. Moreover, several technological and analytical problems and pitfalls that had to be surmounted during our studies focussed on the extensive qualitative and quantitative characterization of salivary peptidoma and mainly based on LC-MS analyses of intact naturally occurring peptides are here described. The hope is that the information provided might be helpful to other groups engaged on the analysis of saliva or other body fluids for clinical applications.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200800100