Enzymatic Ligation Creates Discrete Multinanoparticle Building Blocks for Self-Assembly

Enzymatic ligation of discrete nanoparticle−DNA conjugates creates nanoparticle dimer and trimer structures in which the nanoparticles are linked by single-stranded DNA, rather than by double-stranded DNA as in previous experiments. Ligation was verified by agarose gel and small-angle X-ray scatteri...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2008-07, Vol.130 (29), p.9598-9605
Main Authors: Claridge, Shelley A, Mastroianni, Alexander J, Au, Yeung B, Liang, Huiyang W, Micheel, Christine M, Fréchet, Jean M. J, Alivisatos, A. Paul
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
Chromatography, Ion Exchange - methods
DNA Adducts - chemical synthesis
DNA Adducts - chemistry
DNA Ligases - chemistry
DNA, Single-Stranded
Electrophoresis, Polyacrylamide Gel
Gold - chemistry
Metal Nanoparticles - chemistry
Models, Molecular
Scattering, Small Angle
X-Ray Diffraction
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Summary:Enzymatic ligation of discrete nanoparticle−DNA conjugates creates nanoparticle dimer and trimer structures in which the nanoparticles are linked by single-stranded DNA, rather than by double-stranded DNA as in previous experiments. Ligation was verified by agarose gel and small-angle X-ray scattering. This capability was utilized in two ways: first, to create a new class of multiparticle building blocks for nanoscale self-assembly and, second, to develop a system that can amplify a population of discrete nanoparticle assemblies.
ISSN:0002-7863
1272-7863
1520-5126
DOI:10.1021/ja8026746