Use of Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy To Identify Microbial Metabolic Products on Carbonate Mineral Surfaces

This paper demonstrates the use of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to detect microbial metabolic products on carbonate mineral surfaces. By creating an ATR-FTIR spectral database for specific organic acids using ATR-FTIR spectroscopy we were able to di...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2008-07, Vol.74 (14), p.4553-4559
Main Authors: Bullen, Heather A, Oehrle, Stuart A, Bennett, Ariel F, Taylor, Nicholas M, Barton, Hazel A
Format: Article
Language:English
Subjects:
Acetic Acid - metabolism
Acids
Biological and medical sciences
Calcium Carbonate - chemistry
Chromatography
Chromatography, High Pressure Liquid
E coli
Escherichia coli
Escherichia coli - metabolism
Fourier transforms
Fundamental and applied biological sciences. Psychology
Geomicrobiology
Microbiology
Minerals
Pyruvic Acid - metabolism
Spectroscopy, Fourier Transform Infrared
Spectrum analysis
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Summary:This paper demonstrates the use of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to detect microbial metabolic products on carbonate mineral surfaces. By creating an ATR-FTIR spectral database for specific organic acids using ATR-FTIR spectroscopy we were able to distinguish metabolic acids on calcite surfaces following Escherichia coli growth. The production of these acids by E. coli was verified using high-performance liquid chromatography with refractive index detection. The development of this technique has allowed us to identify microbial metabolic products on carbonate surfaces in nutrient-limited cave environments.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.02936-07