Sub-100 nm IR spectromicroscopy of living cells

We have performed IR spectromicroscopy of cells immersed in liquid water, with a lateral resolution better than 100 nm. Here, we use the motion of an atomic force microscope tip, probing the local transient deformation induced by an IR pulsed laser tuned at a sample absorbing wavelength. By Fourier...

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Bibliographic Details
Published in:Optics letters 2008-07, Vol.33 (14), p.1611-1613
Main Authors: MAYET, C, DAZZI, A, PRAZERES, R, ALLOT, F, GLOTIN, F, ORTEGA, J. M
Format: Article
Language:English
Subjects:
Absorption
Biological and medical sciences
Candida - physiology
Candida - ultrastructure
Equipment Design
Exact sciences and technology
Fourier Analysis
Fundamental and applied biological sciences. Psychology
Fundamental areas of phenomenology (including applications)
General aspects, investigation technics, apparatus
Glycogen - chemistry
Imaging and optical processing
Lasers
Microscopy - instrumentation
Microscopy - methods
Microscopy, Atomic Force
Microspectrophotometry - instrumentation
Microspectrophotometry - methods
Normal Distribution
Optics
Optics and Photonics
Physics
Spectrophotometry, Infrared - instrumentation
Spectrophotometry, Infrared - methods
Spectroscopy, Fourier Transform Infrared
Tissues, organs and organisms biophysics
Water - chemistry
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Summary:We have performed IR spectromicroscopy of cells immersed in liquid water, with a lateral resolution better than 100 nm. Here, we use the motion of an atomic force microscope tip, probing the local transient deformation induced by an IR pulsed laser tuned at a sample absorbing wavelength. By Fourier analysis of the vibration of the cantilever tip, we can discriminate frequencies that are characteristic of the object, thus eliminating the influence of the water absorption. This opens the door of chemical imaging of living species in vivo, with spatial resolution of the order of the size of cell components.
ISSN:0146-9592
1539-4794
DOI:10.1364/ol.33.001611