5-Bromodeoxyuridine Induces Senescence-Like Phenomena in Mammalian Cells Regardless of Cell Type or Species

5-Bromodeoxyuridine was found to induce flat and enlarged cell shape, characteristics of senescent cells, and senescence-associated /J-galactosidase in mammalian cells regardless of cell type or species. In immortal human cells, fibronectin, collagenase I, and p21n/dM mRNAs were immediately and very...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1999-12, Vol.126 (6), p.1052-1059
Main Authors: Eriko, Michishita, Nakabayashi, Kazuhiko, Suzuki, Toshikazu, Kaul, Sunil C., Ogino, Hideki, Fujii, Michihiko, Mitsui, Youji, Ayusawa, Dai
Format: Article
Language:English
Subjects:
5-bromodeoxyuridine
Aging - drug effects
Animals
Biomarkers
Bromodeoxyuridine - pharmacology
Cell Cycle
Cell Line
Electrophoresis, Polyacrylamide Gel
Humans
immortal
Mice
Polymorphism, Restriction Fragment Length
senescence
senescence marker
stress
Telomere
Tumor Cells, Cultured
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:5-Bromodeoxyuridine was found to induce flat and enlarged cell shape, characteristics of senescent cells, and senescence-associated /J-galactosidase in mammalian cells regardless of cell type or species. In immortal human cells, fibronectin, collagenase I, and p21n/dM mRNAs were immediately and very strongly induced, and the mortality marker mortal in changed to the mortal type from the immortal type. Human cell lines lacking functional p2in/.di.i pienn., o r p53 behaved similarly. The protein levels of plG4 and p53 did not change uniformly, while the level of p21‘n/’bl was increased by varying degrees in positive cell lines. Telomerase activity was suppressed in positive cell lines, but accelerated telomere shortening was not observed in tumor cell lines. These results suggest that 5 bromodeoxyuridine activates a common senescence pathway present in both mortal and immortal mammalian cells.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a022549