Derivation of consensus sequence for protein binding site in Yarrowia lipolytica centromere

The transmission of replicative plasmids in the yeast Yarrowia lipolytica is mediated solely by a part of its centromere DNA, in which an essential protein binding site has been analyzed recently. Here, we extended the analysis to other minimized centromeric regions, revealing a consensus sequence o...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2008-06, Vol.105 (6), p.671-674
Main Authors: Yamane, Takayoshi, Ogawa, Takahira, Matsuoka, Masayoshi
Format: Article
Language:English
Subjects:
Base Sequence
Binding Sites
Biological and medical sciences
Biotechnology
Centromere - genetics
centromeres
Consensus Sequence - genetics
DNA replication
DNA, Fungal - genetics
DNA-binding proteins
DNA-Binding Proteins - genetics
Fundamental and applied biological sciences. Psychology
gel mobility shift
high-frequency transformation
Microdissection - methods
Molecular Sequence Data
palindrome sequence
plasmids
Plasmids - genetics
point centromere
Protein Binding
protein binding motif
replicative plasmids
Sequence Analysis, DNA
Yarrowia - genetics
Yarrowia lipolytica
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Summary:The transmission of replicative plasmids in the yeast Yarrowia lipolytica is mediated solely by a part of its centromere DNA, in which an essential protein binding site has been analyzed recently. Here, we extended the analysis to other minimized centromeric regions, revealing a consensus sequence of a 17- to 21-bp imperfect palindrome.
ISSN:1389-1723
1347-4421
DOI:10.1263/jbb.105.671