Magnetic Resonance Imaging Contrast Agent Targeted Toward Activated Platelets Allows In Vivo Detection of Thrombosis and Monitoring of Thrombolysis

Platelets are the key to thrombus formation and play a role in the development of atherosclerosis. Noninvasive imaging of activated platelets would be of great clinical interest. Here, we evaluate the ability of a magnetic resonance imaging (MRI) contrast agent consisting of microparticles of iron o...

Full description

Saved in:
Bibliographic Details
Published in:Circulation (New York, N.Y.) N.Y.), 2008-07, Vol.118 (3), p.258-267
Main Authors: VON ZUR MUHLEN, C, VON ELVERFELDT, D, SCHWARZ, M, BODE, C, PETER, K, MOELLER, J. A, CHOUDHURY, R. P, PAUL, D, HAGEMEYER, C. E, OLSCHEWSKI, M, BECKER, A, NEUDORFER, I, BASSLER, N
Format: Article
Language:English
Subjects:
Animals
Atherosclerosis - diagnosis
Binding Sites
Biological and medical sciences
Blood and lymphatic vessels
Blood. Blood coagulation. Reticuloendothelial system
Cardiology. Vascular system
Carotid Artery Diseases - diagnosis
Contrast Media
Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous
Drug Monitoring - methods
Ferric Compounds
Humans
In Vitro Techniques
Ligands
Magnetic Resonance Imaging
Male
Medical sciences
Mice
Mice, Inbred C57BL
Particle Size
Pharmacology. Drug treatments
Platelet Activation
Platelet Glycoprotein GPIIb-IIIa Complex - metabolism
Thrombolytic Therapy
Thrombosis - blood
Thrombosis - diagnosis
Thrombosis - drug therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Platelets are the key to thrombus formation and play a role in the development of atherosclerosis. Noninvasive imaging of activated platelets would be of great clinical interest. Here, we evaluate the ability of a magnetic resonance imaging (MRI) contrast agent consisting of microparticles of iron oxide (MPIOs) and a single-chain antibody targeting ligand-induced binding sites (LIBS) on activated glycoprotein IIb/IIIa to image carotid artery thrombi and atherosclerotic plaques. Anti-LIBS antibody or control antibody was conjugated to 1-microm MPIOs (LIBS MPIO/control MPIO). Nonocclusive mural thrombi were induced in mice with 6% ferric chloride. MRI (at 9.4 T) was performed once before and repeatedly in 12-minute-long sequences after LIBS MPIO/control MPIO injection. After 36 minutes, a significant signal void, corresponding to MPIO accumulation, was observed with LIBS MPIOs but not control MPIOs (P
ISSN:0009-7322
1524-4539
DOI:10.1161/CIRCULATIONAHA.107.753657