Semiquantitative polymerase chain reaction enzyme immunoassay for the diagnosis of pertussis

The two most commonly used targets for diagnosis of pertussis by the polymerase chain reaction have been the pertussis toxin promoter and the repeated insertion sequence IS481. A comparative assessment of these primers was performed on routinely collected nasopharyngeal swabs, stored at -20 C, using...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 1999-10, Vol.18 (10), p.748-750
Main Authors: MATTHEWS, R. C, GOLBANG, N, BRÜCK, W. M, OWEN, D, BAILEY, A, WESTON, V, KERR, J. R
Format: Article
Language:English
Subjects:
Bacterial diseases
Biological and medical sciences
Bordetella pertussis
Child
DNA - analysis
Ent and stomatologic bacterial diseases
Human bacterial diseases
Humans
Immunoassays
Immunoenzyme Techniques
Infectious diseases
Medical sciences
Nasopharynx - microbiology
Polymerase Chain Reaction - methods
Toxins
Whooping Cough - diagnosis
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Summary:The two most commonly used targets for diagnosis of pertussis by the polymerase chain reaction have been the pertussis toxin promoter and the repeated insertion sequence IS481. A comparative assessment of these primers was performed on routinely collected nasopharyngeal swabs, stored at -20 C, using novel semiquantitative enzyme immunoassays. Both sets of primers behaved similarly with bacterial suspensions, and the 17 culture-positive nasopharyngeal swabs were also positive with the pertussis toxin promoter primers, with one exception, which had been subject to prolonged storage. Significantly more of the 69 culture-negative swabs were positive with the pertussis toxin promoter primers (n = 36) than with the IS481 primers (n = 18). To determine the effect of inhibitors, a comparative assessment of three primer pairs against human DNA (beta-globin and glyceraldehyde-3-phosphate dehydrogenase) was also performed.
ISSN:0934-9723
1435-4373
DOI:10.1007/s100960050392