Determination of bromide in canine plasma using ion chromatography

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-07, Vol.870 (2), p.255-258
• Main Authors: Cox, S.K., Whiton, A.M., Bowman, H.L.
• Format: Article
• Language: English
• Subjects: Administration, Oral; Analysis; Analytical, structural and metabolic biochemistry; Animals; Anticonvulsants - administration & dosage; Anticonvulsants - blood; Anticonvulsants - therapeutic use; Biological and medical sciences; Bromide; Bromides - administration & dosage; Bromides - blood; Bromides - therapeutic use; Chromatography, Ion Exchange - methods; Dog Diseases - blood; Dog Diseases - drug therapy; Dogs; Epilepsy; Epilepsy - drug therapy; Epilepsy - veterinary; Fundamental and applied biological sciences. Psychology; General pharmacology; Ion chromatography; Medical sciences; Pharmacology. Drug treatments; Potassium Compounds - administration & dosage; Potassium Compounds - blood; Potassium Compounds - therapeutic use
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2008.06.023

A new ion chromatographic procedure has been developed and validated for the determination of bromide in canine plasma. Following a simple dilution, samples were separated on a Metrosep A Supp 5 column. The mobile phase was an isocratic mixture of 2.2 mM Na 2CO 3, 1.0 mM NaHCO 3, and 1% acetonitrile, with a flow-rate of 0.7 ml/min. The procedure produced a linear curve over the concentration range of 50–2500 μg/ml. The development of the assay permitted the determination of therapeutic levels after oral administration of potassium bromide to dogs being treated for epilepsy.