Amoxycillin/clavulanic acid combinations increase transmigration of leucocytes through endothelial cell monolayers : endothelial cells play a key role

Postoperative inflammation is still viewed as an unresolved problem. During inflammation, leucocytes play a tremendous role and migrate from intravascular spaces into the tissue to attack microorganisms. Different agents, e.g. anaesthetic drugs, are able to influence leucocyte recruitment. Previous...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 1999-10, Vol.44 (4), p.465-469
Main Authors: HOFBAUER, R, MOSER, D, GMEINER, B, KAYE, A. D, KAPIOTIS, S, WAGNER, O, FRASS, M
Format: Article
Language:English
Subjects:
Amoxicillin-Potassium Clavulanate Combination - blood
Amoxicillin-Potassium Clavulanate Combination - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Cell Movement - drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Drug Therapy, Combination - pharmacology
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Endothelium, Vascular - physiology
Female
Humans
Male
Medical sciences
Neutrophils - drug effects
Neutrophils - physiology
Pharmacology. Drug treatments
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Summary:Postoperative inflammation is still viewed as an unresolved problem. During inflammation, leucocytes play a tremendous role and migrate from intravascular spaces into the tissue to attack microorganisms. Different agents, e.g. anaesthetic drugs, are able to influence leucocyte recruitment. Previous studies have investigated the influence of amoxycillin on chemotaxis of leucocytes alone. The aim of our study was to examine the effect of amoxycillin/clavulanic acid (co-amoxiclav) on leucocyte migration through endothelial cell monolayers (ECMs). Human umbilical endothelial cells were cultured on microporous membranes, achieving a monolayer. Polymorphonuclear neutrophil leucocytes (PMNLs) were used in a migration assay. The numbers of untreated PMNLs migrating through untreated ECMs were used as control and set as 100%. PMNLs and/or ECMs were pretreated with co-amoxiclav using clinically relevant as well as higher and lower concentrations. Co-amoxiclav was able to increase PMNL migration through ECMs significantly (P
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/44.4.465