Ovocleidin (OC 116) Is Present in Avian Skeletal Tissues

Ovocleidin (OC-116), a protein identified in eggshell matrix, was found to be expressed in avian growth plate chondrocytes. Because OC-116 has been reported to be a member of a family of related phosphoprotein genes clustered on avian chromosome 4, we expanded our search to other skeletal tissues. U...

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Bibliographic Details
Published in:Poultry science 2008-08, Vol.87 (8), p.1618-1623
Main Authors: Horvat-Gordon, M, Yu, F, Burns, D, Leach, R.M. Jr
Format: Article
Language:English
Subjects:
animal proteins
Animals
Blotting, Western
bone matrix
bones
chickens
Chickens - genetics
Chickens - metabolism
chicks
chondrocytes
Chondrocytes - metabolism
chromosome mapping
Egg Proteins - biosynthesis
Egg Proteins - genetics
Egg Shell - metabolism
egg shell matrix
egg shell proteins
Female
genes
growth plate
Growth Plate - metabolism
hypertrophy
laying hens
multigene family
ovocleidin 116
phosphoproteins
reverse transcriptase polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA - chemistry
RNA - genetics
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Summary:Ovocleidin (OC-116), a protein identified in eggshell matrix, was found to be expressed in avian growth plate chondrocytes. Because OC-116 has been reported to be a member of a family of related phosphoprotein genes clustered on avian chromosome 4, we expanded our search to other skeletal tissues. Using Western blotting, we found OC-116 in the matrix of chick cortical bone and laying hen medullary bone as well as in hypertrophic chondrocyte lysates. Furthermore, other members of this family (bone sialoprotein, dental matrix protein-1, and osteopontin) were also present in the eggshell matrix. Reverse transcription-PCR was used to confirm the presence of the OC-116 gene in bone tissues as well as the expression of bone sialoprotein and dental matrix protein-1 in uterine tissue. These results, in combination with those of other laboratories, show that this family of phosphoproteins is found in a wide variety of avian mineralized tissues.
ISSN:0032-5791
1525-3171
DOI:10.3382/ps.2008-00031