Peroxisomes as sites for synthesis of polyhydroxyalkanoates in transgenic plants

Bacterial genes responsible for poly(3‐hydroxybutyrate) (PHB) biosynthesis were targeted to plant peroxisomes by adding a carboxy‐terminal targeting sequence. The enzymes evidently were transported into peroxisomes, retained their catalytic activity, and reacted with peroxisomally available precurso...

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Bibliographic Details
Published in:Biotechnology progress 1999-11, Vol.15 (6), p.1053-1057
Main Authors: Hahn, J.J, Eschenlauer, A.C, Sleytr, U.B, Somers, D.A, Srienc, F
Format: Article
Language:English
Subjects:
Bacteria
biolistics
Biological and medical sciences
Biosynthesis
Biotechnology
Biotechnology - methods
Catalyst activity
Cells, Cultured
Enzymes
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
Genetic engineering
Genetic technics
genetic transformation
Hydroxybutyrates - chemical synthesis
Hydroxybutyrates - metabolism
Methods. Procedures. Technologies
Peroxisomes - metabolism
Plant cell culture
Plants, Genetically Modified - metabolism
Polyesters
Polyesters - chemical synthesis
Polyesters - metabolism
polyhydroxybutyrate
polyhydroxybutyric acid
Transgenic animals and transgenic plants
Transgenic plants
Zea mays
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Summary:Bacterial genes responsible for poly(3‐hydroxybutyrate) (PHB) biosynthesis were targeted to plant peroxisomes by adding a carboxy‐terminal targeting sequence. The enzymes evidently were transported into peroxisomes, retained their catalytic activity, and reacted with peroxisomally available precursors because PHB synthesis in transgenic plant cells was localized to peroxisomes. Up to 2 mg/g fresh weight PHB was produced in suspension cultures of Black Mexican Sweet maize cells after biolistic transformation with three peroxisomally targeted bacterial genes. An equilibrium effect is proposed to explain the unexpected existence of (R)‐3‐hydroxybutyryl‐CoA in plant peroxisomes.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp990118n