Influence of Transgenic Overexpression of Phospholamban on Postextrasystolic Potentiation

Twelve mice with PLB overexpression (PLBOE), and 11 isogenic FVB/N wild-type (WT) controls, were anesthetized and instrumented with a 1.4 F Millar catheter in the LV and a 1 F pacemaker in the right atrium. At a cycle length of 200 ms and a fixed extrastimulus of 120 ms, extrastimuli with increasing...

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Bibliographic Details
Published in:Journal of molecular and cellular cardiology 1999-11, Vol.31 (11), p.2007-2015
Main Authors: Hoit, Brian D., Tramuta, Daniel A., Kadambi, Vivek J., Dash, Raj, Ball, Nancy, Kranias, Evangelia G., Walsh, Richard A.
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
Animals
Ca2+ATPase
Calcium-Binding Proteins - deficiency
Calcium-Binding Proteins - genetics
Calcium-Binding Proteins - physiology
Cardiac Complexes, Premature - genetics
Cardiac Complexes, Premature - physiopathology
Diastole
Electric Stimulation
Electrocardiography
Female
Force-interval behavior
Heart - drug effects
Heart - physiology
Heart - physiopathology
Hemodynamics
Male
Mice
Mice, Knockout
Mice, Transgenic
Phospholamban
Ryanodine - pharmacology
Sarcoplasmic reticulum
Systole
Ventricular Function, Left
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Summary:Twelve mice with PLB overexpression (PLBOE), and 11 isogenic FVB/N wild-type (WT) controls, were anesthetized and instrumented with a 1.4 F Millar catheter in the LV and a 1 F pacemaker in the right atrium. At a cycle length of 200 ms and a fixed extrastimulus of 120 ms, extrastimuli with increasing intervals (PESI) up to 1000 ms were introduced, and the peak rates of LV isovolumic contraction (±dP/dtmax) were normalized and fit to monoexponential equations. In a subset of animals, the protocols were repeated after ryanodine (4 ng/g) was given to deplete SR Ca2+stores. The time constant and the plateau of the exponential curve fits were significantly greater in PLBOE than WT (107.8±7.0v 75.2±5.5 ms and 1.39±0.03 v 1.08±0.02, both P
ISSN:0022-2828
1095-8584
DOI:10.1006/jmcc.1999.1031