A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines

In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1α (IL1α) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to...

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Bibliographic Details
Published in:Journal of immunological methods 1999-11, Vol.230 (1), p.19-27
Main Authors: Spagnuolo-Weaver, M, Fuerst, R, Campbell, S.T, Meehan, B.M, McNeilly, F, Adair, B, Allan, G
Format: Article
Language:English
Subjects:
Actins - genetics
Animals
Biological and medical sciences
Cytokines
Cytokines - analysis
Cytokines - genetics
DNA Primers - genetics
DNA, Complementary - genetics
Evaluation Studies as Topic
Fluorimeter-based PCR
Fluorometry - methods
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression
interleukin 1^a
interleukin 1a
Interleukin-1 - analysis
Interleukin-1 - genetics
Interleukin-2 - analysis
Interleukin-2 - genetics
Leukocytes - immunology
Molecular immunology
PCR
Plasmids - genetics
Porcine
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - analysis
RNA, Messenger - genetics
Swine
Techniques
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Description
Summary:In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1α (IL1α) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to a standard PCR performed in a block-based thermocycler.
ISSN:0022-1759
1872-7905
DOI:10.1016/S0022-1759(99)00114-3