A serosurvey for hantavirus infection in wild rodents from the states of Rio de Janeiro and Pernambuco, Brazil

Sera from 269 rodents obtained during the routine surveillance operations in plague areas of Rio de Janeiro and Pernambuco states, Brazil were tested by ELISA for specific IgG antibodies against a recombinant nucleocapsid (N) protein of Araraquara hantavirus. ELISA-positive sera were submitted to re...

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Bibliographic Details
Published in:Acta tropica 2008-08, Vol.107 (2), p.150-152
Main Authors: Sobreira, M., Souza, G.T., Moreli, M.L., Borges, A.A., Morais, F.A., Figueiredo, L.T.M., Almeida, A.M.P.
Format: Article
Language:English
Subjects:
Animals
Animals, Wild - classification
Animals, Wild - virology
Antibodies, Viral - blood
Bacterial diseases
Biological and medical sciences
Brazil - epidemiology
Enzyme-Linked Immunosorbent Assay
General aspects
Hantavirus
Hantavirus - classification
Hantavirus - genetics
Hantavirus - immunology
Hantavirus - isolation & purification
Hantavirus Infections - epidemiology
Hantavirus Infections - veterinary
Hantavirus Infections - virology
Hantaviruses
Human bacterial diseases
Immunoglobulin G - blood
Infectious diseases
Medical sciences
Plague
Reverse Transcriptase Polymerase Chain Reaction
Rodent Diseases - epidemiology
Rodent Diseases - virology
Rodents
Sigmodontinae - classification
Sigmodontinae - virology
Tropical bacterial diseases
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Description
Summary:Sera from 269 rodents obtained during the routine surveillance operations in plague areas of Rio de Janeiro and Pernambuco states, Brazil were tested by ELISA for specific IgG antibodies against a recombinant nucleocapsid (N) protein of Araraquara hantavirus. ELISA-positive sera were submitted to reverse transcriptase-polymerase chain reaction (RT-PCR) for amplification of the virus genome and later sequencing for identification of the viral variant. The samples from the state of Pernambuco were antibody negative, and although four from Rio de Janeiro were ELISA-positive, they failed to yield viral cDNA by RT-PCR. This is the first report of the presence of antibodies to a hantavirus among rodents from Rio de Janeiro and suggests the possibility of human cases of hantavirus pulmonary syndrome (HPS) in that state, although no case has yet been reported.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2008.05.018