HGF triggers activation of the COX‐2 gene in rat gastric epithelial cells: action mediated through the ERK2 signaling pathway

• Published in: The FASEB journal 1999-12, Vol.13 (15), p.2186-2194
• Main Authors: JONES, MICHAEL K., SASAKI, EIJI, HALTER, FRED, PAI, RAMA, NAKAMURA, TOSHIKAZU, ARAKAWA, TETSUO, KUROKI, TETSUO, TARNAWSKI, ANDRZEJ S.
• Format: Article
• Language: English
• Subjects: Animals; Cell Survival; Cells, Cultured; COX-2 gene; Cyclooxygenase 1; Cyclooxygenase 2; c‐Met/HGF receptor; ERK2 protein; Gastric Mucosa - metabolism; hepatocyte growth factor; Hepatocyte Growth Factor - metabolism; Isoenzymes - biosynthesis; Isoenzymes - genetics; Isoenzymes - metabolism; MAP Kinase Signaling System; Membrane Proteins; Mitogen-Activated Protein Kinase 1 - metabolism; Phosphorylation; Prostaglandin-Endoperoxide Synthases - biosynthesis; Prostaglandin-Endoperoxide Synthases - genetics; Prostaglandin-Endoperoxide Synthases - metabolism; Proto-Oncogene Proteins c-met - metabolism; Rats; receptor phosphorylation; Reverse Transcriptase Polymerase Chain Reaction; RGM1 cells; RNA, Messenger - metabolism; Stomach Ulcer - enzymology; Stomach Ulcer - metabolism
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.13.15.2186

ABSTRACT Although it is established that growth factors and prostaglandins function in the maintenance of gastric mucosal integrity and in the healing of gastric mucosal injury and ulceration, the regulatory relationship between growth factors and prostaglandins in the gastric mucosa is not well characterized. Therefore, we investigated whether hepatocyte growth factor (HGF) affects expression of COX‐2 (the inducible form of the prostaglandin synthesizing enzyme, cyclooxygenase) in gastric epithelial cells and whether this action is mediated through the MAP (ERK) kinase signaling pathway. In RGM1 cells (an epithelial cell line derived from normal rat gastric mucosa), HGF caused an increase in COX‐2 mRNA and protein by 236% and 175%, respectively (both P