Plasma Levels of Soluble Tie2 and Vascular Endothelial Growth Factor Distinguish Critical Limb Ischemia From Intermittent Claudication in Patients With Peripheral Arterial Disease

• Published in: Journal of the American College of Cardiology 2008-07, Vol.52 (5), p.387-393
• Main Authors: Findley, Clarence M., PhD, Mitchell, Robert G., MD, Duscha, Brian D., MS, Annex, Brian H., MD, Kontos, Christopher D., MD, FACC
• Format: Article
• Language: English
• Subjects: Acute coronary syndromes; Adult; Aged; Aged, 80 and over; Analysis of Variance; Angiogenesis; Angiopoietin-2 - blood; Biological and medical sciences; Biomarkers; Biomarkers - blood; Blood and lymphatic vessels; Cardiology; Cardiology. Vascular system; Cardiovascular; Cardiovascular disease; Cell culture; critical limb ischemia; Demographics; Diabetes; Diagnosis, Differential; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Enzymes; Female; Humans; Intermittent Claudication - blood; Intermittent Claudication - diagnosis; Internal Medicine; Ischemia; Ischemia - blood; Ischemia - diagnosis; Lower Extremity - blood supply; Male; Medical sciences; Middle Aged; Mortality; Pain; Patients; peripheral arterial disease; Peripheral Vascular Diseases - blood; Placenta Growth Factor; Plasma; Pregnancy Proteins - blood; Proteins; Receptor, TIE-2 - blood; Receptors, Vascular Endothelial Growth Factor - blood; soluble Tie2; Studies; Variance analysis; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - blood
• ISSN: 0735-1097; 1558-3597
• DOI: 10.1016/j.jacc.2008.02.045

Plasma Levels of Soluble Tie2 and Vascular Endothelial Growth Factor Distinguish Critical Limb Ischemia From Intermittent Claudication in Patients With Peripheral Arterial Disease Clarence M. Findley, Robert G. Mitchell, Brian D. Duscha, Brian H. Annex, Christopher D. Kontos We examined changes in factors that regulate angiogenesis in patients with peripheral arterial disease (PAD) and found that plasma angiopoietin-2, vascular endothelial growth factor (VEGF), and soluble Tie2 (sTie2) were significantly increased in patients with PAD. Levels of VEGF and sTie2 were significantly increased in patients with critical limb ischemia (CLI) independent of cardiovascular risk factors or the ankle-brachial index, and VEGF induced sTie2 release from endothelial cells in vitro. These findings suggest that VEGF and sTie2 may provide novel biomarkers of CLI and that sTie2 may play a causal role in this manifestation of PAD.