Postnatal development of cyclooxygenase-2 in the rat kidney

Prostaglandins are local mediators/modulators of kinin effects in the kidney. The prostaglandin G2/H2 synthase (cyclooxygenase, COX) is the key regulatory enzyme of prostanoid synthesis pathway. Two COX isoenzymes (constitutive or COX-1 and inducible or COX-2) have been described in the rat kidney....

Full description

Saved in:
Bibliographic Details
Published in:Immunopharmacology 1999-10, Vol.44 (1-2), p.205-210
Main Authors: Vio, Carlos P., Balestrini, Claudia, Recabarren, Monica, Cespedes, Carlos
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn - growth & development
Animals, Newborn - metabolism
Biological and medical sciences
Cyclooxygenase 2
Fundamental and applied biological sciences. Psychology
Gene expression
glucocorticoids
Immunoenzyme Techniques
Immunohistochemistry
Isoenzymes - metabolism
Kidney - enzymology
Kidney - growth & development
Molecular and cellular biology
Molecular genetics
Peroxidases
Prostaglandin-Endoperoxide Synthases - metabolism
Rats
Rats, Sprague-Dawley
Renal development
Thick ascending limb of Henle
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Prostaglandins are local mediators/modulators of kinin effects in the kidney. The prostaglandin G2/H2 synthase (cyclooxygenase, COX) is the key regulatory enzyme of prostanoid synthesis pathway. Two COX isoenzymes (constitutive or COX-1 and inducible or COX-2) have been described in the rat kidney. We have demonstrated the presence of COX-2 in a subset of thick ascending limb of Henle (TAL) cells in normal adult rats [Vio, C.P., Cespedes, C., Gallardo, P., Masferrer, J.L., 1997. Renal identification of cyclooxygenase-2 in a subset of thick ascending limb cells. Hypertension 30, 687–692]. The present work was designed to evaluate COX-2 during the postnatal development of the rat kidney. Kidneys from Sprague–Dawley rats were studied during postnatal days 5, 10, 15 days and adult (60 days) (n=8 each group). Renal tissue was immunostained with specific antibodies against COX-2. COX-2 was observed exclusively in TAL. A small number of COX-2 cells were observed during early postnatal life, increasing from day 5 to 15, and decreasing thereafter to reach adult levels. During maximal expression, near 20% of TAL were COX-2 positive whereas in early postnatal period and adults, only 2% of TAL cells contain COX-2. This transient induction of COX-2 during development suggest that the enzyme is necessary for the postnatal development of the kidney. This change in COX-2 seems to correspond to a derepression of COX-2 gene expression secondary to low levels of glucocorticoids.
ISSN:0162-3109
DOI:10.1016/S0162-3109(99)00091-0