High-speed gas chromatography: The importance of instrumentation optimization and the elimination of extra-column band broadening

This review provides a summary of chromatographic theory as it applies to high-speed gas chromatography. A novel method for determining the optimal linear flow velocity, u ¯ opt , from specific experimental parameters, is discussed. An in-depth theoretical understanding of u ¯ opt and its relation t...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 2008-08, Vol.76 (4), p.703-717
Main Authors: Reid, Vanessa R., Synovec, Robert E.
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Chemical separations
Chemistry
Chemotactic Factors - analysis
Chemotactic Factors - isolation & purification
Chromatographic methods and physical methods associated with chromatography
Chromatography, Gas - methods
Diffusion
Equipment Design
Exact sciences and technology
Gas chromatographic methods
Gas chromatographic theory
Gas chromatography
General, instrumentation
High-speed
Insecta
Instrumentation advancements
Microscopy, Electron, Scanning
Models, Theoretical
Optimal linear flow velocity
Pressure
Temperature
Time Factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This review provides a summary of chromatographic theory as it applies to high-speed gas chromatography. A novel method for determining the optimal linear flow velocity, u ¯ opt , from specific experimental parameters, is discussed. An in-depth theoretical understanding of u ¯ opt and its relation to experimental parameters is presented, in the absence of extra-column band broadening, as a means of method evaluation and optimization. Recent developments in high-speed GC are discussed, in the context of the theory presented within this review, to ascertain the influence of extra-column band broadening. The theory presented herein can be used as a means of evaluating the various areas of GC instrumentation (injection, separation, detection, etc.) that need further development to further minimize the effects of extra-column band broadening. The theoretical framework provided in this review, can be, and is, readily used to evaluate high-speed GC results presented in the literature, and thus, the general practitioner may more readily select a specific capillary length and/or internal diameter for a given application. For example, it is theoretically shown, and prior work cited, that demonstrates a peak width of ∼1 ms is readily achievable in GC, when extra-column band broadening is eliminated.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2008.05.012