Oral administration recombinant porcine epidermal growth factor enhances the jejunal digestive enzyme genes expression and activity of early-weaned piglets

• Published in: Journal of animal physiology and animal nutrition 2008-08, Vol.92 (4), p.463-470
• Main Authors: Lee, D.N, Chuang, Y.S, Chiou, H.Y, Wu, F.Y, Yen, H.T, Weng, C.F
• Format: Article
• Language: English
• Subjects: Administration, Oral; Alkaline Phosphatase - metabolism; alpha-Glucosidases - metabolism; Animals; Digestion - drug effects; Digestion - physiology; digestive enzymes; Dose-Response Relationship, Drug; epidermal growth factor; Epidermal Growth Factor - administration & dosage; Epidermal Growth Factor - pharmacology; gene expression; Gene Expression Regulation, Enzymologic; Intestinal Mucosa - drug effects; Jejunum - enzymology; Jejunum - growth & development; Jejunum - metabolism; Lactase - metabolism; Pepsin A - metabolism; pig; Random Allocation; RNA, Messenger - metabolism; Stomach - enzymology; Sucrase - metabolism; Swine; Weaning
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/j.1439-0396.2007.00735.x

This study attempted to determine ingested porcine epidermal growth factor (pEGF) on the gastrointestinal tract development of early-weaned piglets. Thirty-two piglets (14-day weaned) were randomly allotted to supplemented with 0 (control), 0.5, 1.0, or 1.5 mg pEGF/kg diet. Each treatment consisted of four replicates with two pigs per pen for a 14 days experimental period. Piglets were sacrificed and gastrointestinal tract samples were collected to measure mucosa morphology, mRNA expression and activities of digestive enzymes in the gastrointestinal tract of piglets at the end of the experiment. Diets supplemented with pEGF failed to influence growth performance but tended to increase jejunal mucosa weight (p < 0.09) and protein content (p < 0.07). Piglets supplemental pEGF induced incrementally the gastric pepsin activity (p < 0.05) and stimulated jejunal alkaline phosphatase (ALP) and lactase activities accompanied with the increase of jejunal ALP and maltase mRNA expression. No effect of pEGF on the activities of all enzymes in ileum except the stimulation of ileal aminopeptide N mRNA expression. These results reveal that dietary pEGF supplementation might enhance gene expression and activities of digestive enzymes in the stomach and jejunum of piglets.