Protection of Respiratory Chain Enzymes from Ischaemic Damage in Adult Rat Brain Slices

The effect of aglycaemic hypoxia (AH) on the activity of the mitochondrial respiratory chain complexes was measured in superfused adult cortical brain slices. After 15 min of AH the activity of complex II–III was significantly reduced (by 45%) with no change in complex I or IV. Following 30 min of r...

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Bibliographic Details
Published in:Neurochemical research 2008-09, Vol.33 (9), p.1711-1716
Main Authors: Brooks, Keith J., Hargreaves, Iain P., Bates, Timothy E.
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Brain Ischemia - metabolism
Brain Ischemia - pathology
Cell Biology
Citrate (si)-Synthase - metabolism
Electron Transport - physiology
Electron Transport Complex I - metabolism
Electron Transport Complex II - metabolism
Electron Transport Complex III - metabolism
Electron Transport Complex IV - metabolism
Female
Male
Mitochondria - metabolism
Neurochemistry
Neurology
Neurosciences
Original Paper
Rats
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Summary:The effect of aglycaemic hypoxia (AH) on the activity of the mitochondrial respiratory chain complexes was measured in superfused adult cortical brain slices. After 15 min of AH the activity of complex II–III was significantly reduced (by 45%) with no change in complex I or IV. Following 30 min of reperfusion the activities of complex II–III and IV were significantly reduced (by 45% and 20% respectively). These reductions in enzyme activity were abolished by removing the external calcium or by the addition of Nω-nitro- l -arginine (LNNA) or an analogue of superoxide dismutase (SOD) manganese [III] tetrakis 4-benzoic acid porphyrin (Mn-TBAP). These data suggest that a reactive oxygen species (ROS) such as peroxynitrite is involved in the reduction of mitochondrial complex activities following AH.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-008-9609-9