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In vitro infectivity of species of Leishmania (Viannia) responsible for American cutaneous leishmaniasis
There is little available information regarding the infectivity of New World Leishmania species, particularly those from the Amazonian Brazil, where there are six species of the subgenus Viannia causing American cutaneous leishmaniasis (ACL). The aim of this study was to compare, in vitro, the poten...
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| Published in: | Parasitology research (1987) 2008-09, Vol.103 (4), p.771-776 |
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| container_title | Parasitology research (1987) |
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| creator | Campos, Marliane Batista De Castro Gomes, Cláudia Maria de Souza, Adelson Alcimar Almeida Lainson, Ralph Corbett, Carlos Eduardo Pereira Silveira, Fernando Tobias |
| description | There is little available information regarding the infectivity of New World Leishmania species, particularly those from the Amazonian Brazil, where there are six species of the subgenus Viannia causing American cutaneous leishmaniasis (ACL). The aim of this study was to compare, in vitro, the potential infectivity of the following Leishmania (Viannia) spp.: L. (V.) braziliensis from localized cutaneous leishmaniasis (LCL) and mucocutaneous leishmaniasis (MCL) patients, L. (V.) guyanensis, L. (V.) shawi, L. (V.) lainsoni and L. (V.) naiffi from LCL patients only, in cultured BALB/c mice peritoneal macrophage, as well as the production of NO by the infected cells. The infectivity of parasites was expressed by the infection index and, the nitric oxide (NO) production in the macrophage culture supernatant was measured by the Griess method. It was found that L. (V.) braziliensis from MCL, the more severe form of disease, showed the highest (p |
| doi_str_mv | 10.1007/s00436-008-1039-8 |
| format | article |
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The aim of this study was to compare, in vitro, the potential infectivity of the following Leishmania (Viannia) spp.: L. (V.) braziliensis from localized cutaneous leishmaniasis (LCL) and mucocutaneous leishmaniasis (MCL) patients, L. (V.) guyanensis, L. (V.) shawi, L. (V.) lainsoni and L. (V.) naiffi from LCL patients only, in cultured BALB/c mice peritoneal macrophage, as well as the production of NO by the infected cells. The infectivity of parasites was expressed by the infection index and, the nitric oxide (NO) production in the macrophage culture supernatant was measured by the Griess method. It was found that L. (V.) braziliensis from MCL, the more severe form of disease, showed the highest (p <= 0.05) infection index (397), as well as the lowest NO production (2.15 μM) compared with those of other species. In contrast, L. (V.) naiffi which is less pathogenic for the human showed the lowest infection index (301) and the highest NO production (4.11 μM). These results demonstrated a negative correlation between the infectivity and the ability of these parasites to escape from the microbicidal activity of the host cell.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-008-1039-8</identifier><identifier>PMID: 18528708</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Animals ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Cells, Cultured ; Culture Media - chemistry ; Female ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Humans ; Immunology ; Invertebrates ; Leishmania - immunology ; Leishmania - isolation & purification ; Leishmania - pathogenicity ; Leishmaniasis, Cutaneous - immunology ; Leishmaniasis, Cutaneous - parasitology ; Macrophages, Peritoneal - immunology ; Macrophages, Peritoneal - parasitology ; Male ; Medical Microbiology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Nitric Oxide - biosynthesis ; Original Paper</subject><ispartof>Parasitology research (1987), 2008-09, Vol.103 (4), p.771-776</ispartof><rights>Springer-Verlag 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-d24a5817befd168ddac4e958877c146aae6f669ed03462093865a2a514917fcd3</citedby><cites>FETCH-LOGICAL-c396t-d24a5817befd168ddac4e958877c146aae6f669ed03462093865a2a514917fcd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20558479$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18528708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Campos, Marliane Batista</creatorcontrib><creatorcontrib>De Castro Gomes, Cláudia Maria</creatorcontrib><creatorcontrib>de Souza, Adelson Alcimar Almeida</creatorcontrib><creatorcontrib>Lainson, Ralph</creatorcontrib><creatorcontrib>Corbett, Carlos Eduardo Pereira</creatorcontrib><creatorcontrib>Silveira, Fernando Tobias</creatorcontrib><title>In vitro infectivity of species of Leishmania (Viannia) responsible for American cutaneous leishmaniasis</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>There is little available information regarding the infectivity of New World Leishmania species, particularly those from the Amazonian Brazil, where there are six species of the subgenus Viannia causing American cutaneous leishmaniasis (ACL). The aim of this study was to compare, in vitro, the potential infectivity of the following Leishmania (Viannia) spp.: L. (V.) braziliensis from localized cutaneous leishmaniasis (LCL) and mucocutaneous leishmaniasis (MCL) patients, L. (V.) guyanensis, L. (V.) shawi, L. (V.) lainsoni and L. (V.) naiffi from LCL patients only, in cultured BALB/c mice peritoneal macrophage, as well as the production of NO by the infected cells. The infectivity of parasites was expressed by the infection index and, the nitric oxide (NO) production in the macrophage culture supernatant was measured by the Griess method. It was found that L. (V.) braziliensis from MCL, the more severe form of disease, showed the highest (p <= 0.05) infection index (397), as well as the lowest NO production (2.15 μM) compared with those of other species. In contrast, L. (V.) naiffi which is less pathogenic for the human showed the lowest infection index (301) and the highest NO production (4.11 μM). These results demonstrated a negative correlation between the infectivity and the ability of these parasites to escape from the microbicidal activity of the host cell.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cells, Cultured</subject><subject>Culture Media - chemistry</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Humans</subject><subject>Immunology</subject><subject>Invertebrates</subject><subject>Leishmania - immunology</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmania - pathogenicity</subject><subject>Leishmaniasis, Cutaneous - immunology</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Macrophages, Peritoneal - immunology</subject><subject>Macrophages, Peritoneal - parasitology</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Original Paper</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kE1vFSEUhonR2Gv1B7hRNhpdjB6Gj4Fl06htcpMuat0SLgMtzQxz5cw06b-Xm7mpu654Cc97gIeQ9wy-MYDuOwIIrhoA3TDgptEvyIYJ3jbMSPmSbMDUDIzxE_IG8R6AdUqI1-SEadnqDvSG3F1m-pDmMtGUY_BzqptHOkWK--BTwEPchoR3o8vJ0S9_kss1fKUl4H7KmHZDoHEq9GwMJXmXqV9ml8O0IB2eepjwLXkV3YDh3XE9JTc_f_w-v2i2V78uz8-2jedGzU3fCic163Yh9kzpvndeBCO17jrPhHIuqKiUCT1wodr6Qa2ka51kwrAu-p6fks_r3H2Z_i4BZzsm9GEY1kdZZbgSUqgKshX0ZUIsIdp9SaMrj5aBPei1q15b9dqDXqtr58Nx-LIbQ_-_cfRZgU9HwKF3Qywu-4RPXAtSatGZyrUrh_Uo34Zi76el5Crm2ds_rqXoJutuSx18c90C4wCmBdUJ_g_eWJxY</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Campos, Marliane Batista</creator><creator>De Castro Gomes, Cláudia Maria</creator><creator>de Souza, Adelson Alcimar Almeida</creator><creator>Lainson, Ralph</creator><creator>Corbett, Carlos Eduardo Pereira</creator><creator>Silveira, Fernando Tobias</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>In vitro infectivity of species of Leishmania (Viannia) responsible for American cutaneous leishmaniasis</title><author>Campos, Marliane Batista ; De Castro Gomes, Cláudia Maria ; de Souza, Adelson Alcimar Almeida ; Lainson, Ralph ; Corbett, Carlos Eduardo Pereira ; Silveira, Fernando Tobias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-d24a5817befd168ddac4e958877c146aae6f669ed03462093865a2a514917fcd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cells, Cultured</topic><topic>Culture Media - chemistry</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Humans</topic><topic>Immunology</topic><topic>Invertebrates</topic><topic>Leishmania - immunology</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmania - pathogenicity</topic><topic>Leishmaniasis, Cutaneous - immunology</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Macrophages, Peritoneal - immunology</topic><topic>Macrophages, Peritoneal - parasitology</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Original Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Campos, Marliane Batista</creatorcontrib><creatorcontrib>De Castro Gomes, Cláudia Maria</creatorcontrib><creatorcontrib>de Souza, Adelson Alcimar Almeida</creatorcontrib><creatorcontrib>Lainson, Ralph</creatorcontrib><creatorcontrib>Corbett, Carlos Eduardo Pereira</creatorcontrib><creatorcontrib>Silveira, Fernando Tobias</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Campos, Marliane Batista</au><au>De Castro Gomes, Cláudia Maria</au><au>de Souza, Adelson Alcimar Almeida</au><au>Lainson, Ralph</au><au>Corbett, Carlos Eduardo Pereira</au><au>Silveira, Fernando Tobias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro infectivity of species of Leishmania (Viannia) responsible for American cutaneous leishmaniasis</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>103</volume><issue>4</issue><spage>771</spage><epage>776</epage><pages>771-776</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>There is little available information regarding the infectivity of New World Leishmania species, particularly those from the Amazonian Brazil, where there are six species of the subgenus Viannia causing American cutaneous leishmaniasis (ACL). The aim of this study was to compare, in vitro, the potential infectivity of the following Leishmania (Viannia) spp.: L. (V.) braziliensis from localized cutaneous leishmaniasis (LCL) and mucocutaneous leishmaniasis (MCL) patients, L. (V.) guyanensis, L. (V.) shawi, L. (V.) lainsoni and L. (V.) naiffi from LCL patients only, in cultured BALB/c mice peritoneal macrophage, as well as the production of NO by the infected cells. The infectivity of parasites was expressed by the infection index and, the nitric oxide (NO) production in the macrophage culture supernatant was measured by the Griess method. It was found that L. (V.) braziliensis from MCL, the more severe form of disease, showed the highest (p <= 0.05) infection index (397), as well as the lowest NO production (2.15 μM) compared with those of other species. In contrast, L. (V.) naiffi which is less pathogenic for the human showed the lowest infection index (301) and the highest NO production (4.11 μM). These results demonstrated a negative correlation between the infectivity and the ability of these parasites to escape from the microbicidal activity of the host cell.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18528708</pmid><doi>10.1007/s00436-008-1039-8</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Cells, Cultured Culture Media - chemistry Female Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Humans Immunology Invertebrates Leishmania - immunology Leishmania - isolation & purification Leishmania - pathogenicity Leishmaniasis, Cutaneous - immunology Leishmaniasis, Cutaneous - parasitology Macrophages, Peritoneal - immunology Macrophages, Peritoneal - parasitology Male Medical Microbiology Mice Mice, Inbred BALB C Microbiology Nitric Oxide - biosynthesis Original Paper |
| title | In vitro infectivity of species of Leishmania (Viannia) responsible for American cutaneous leishmaniasis |
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