Two-step purification of low-content cellular protein using protein-imprinted polymers

We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namel...

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Bibliographic Details
Published in:Analytical biochemistry 2008-09, Vol.380 (2), p.268-275
Main Authors: Long, Yi, Xing, Xiaocui, Han, Ruifang, Sun, Yang, Wang, Ying, Zhao, Zhuo, Mi, Huaifeng
Format: Article
Language:English
Subjects:
Animals
Assistant recognition polymer chains (ARPCs)
Cell Extracts - analysis
Cloned bacterial protein as template
Liver - chemistry
Microspheres
Molecular Imprinting - methods
Polyethylene Glycols - chemistry
Polymers - chemical synthesis
Polymers - chemistry
Polyvinyl Alcohol - chemical synthesis
Polyvinyl Alcohol - chemistry
Protein-imprinted polymer
Proteins - isolation & purification
Purification of cellular protein
RNA, Messenger - chemistry
RNA, Messenger - genetics
Swine
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Summary:We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2008.05.045