Preliminary study of the incidence of disomy in sperm fractions after MicroSort flow cytometry

Using triple colour fluorescent in-situ hybridization (FISH) we have evaluated, on a blind basis, the disomy level for chromosome 21 and the sex chromosomes in flow cytometric sorted sperm samples. There were no statistically significant differences in the disomy rates when comparing the sorted samp...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1999-12, Vol.14 (12), p.2987-2990
Main Authors: Vidal, F., Blanco, J., Fugger, E.F., Keyvanfar, K., Norton, M., Schulman, J.D., Egozcue, J.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cell Separation
chromosome 21 disomies
Chromosomes, Human, Pair 21 - genetics
Diploidy
Flow Cytometry
fluorescence in-situ hybridization
Fundamental and applied biological sciences. Psychology
Humans
In Situ Hybridization, Fluorescence
Incidence
Male
Mammalian male genital system
Morphology. Physiology
Sex Chromosomes - genetics
sperm sex selection
Spermatozoa - physiology
Vertebrates: reproduction
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Summary:Using triple colour fluorescent in-situ hybridization (FISH) we have evaluated, on a blind basis, the disomy level for chromosome 21 and the sex chromosomes in flow cytometric sorted sperm samples. There were no statistically significant differences in the disomy rates when comparing the sorted samples (either for X- or Y-bearing spermatozoa) with non-sorted samples. There were no diploid spermatozoa observed in any sample group after MicroSort® processing.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/14.12.2987