Bovine neosporosis: comparison of serological methods using outbreak sera from a dairy herd in New Zealand

Serological methods developed to detect Neospora caninum specific bovine antibodies were compared using sera from an abortion outbreak on a dairy farm in New Zealand. These methods included four ELISAs, IFAT and immunoblot. The abortions ( n=16) on the farm were restricted to one of two groups of th...

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Bibliographic Details
Published in:International journal for parasitology 1999-10, Vol.29 (10), p.1659-1667
Main Authors: Schares, G, Conraths, F.J, Reichel, M.P
Format: Article
Language:English
Subjects:
Abortion
Abortion, Veterinary - epidemiology
Abortion, Veterinary - parasitology
Animals
antibodies
Antibodies, Protozoan - blood
Apicomplexa
calves
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - parasitology
Coccidiosis - epidemiology
Coccidiosis - parasitology
Coccidiosis - veterinary
Commercial test
Cut-off
dairy cows
dairy herds
Dairying
Disease Outbreaks - veterinary
ELISA
enzyme-linked immunosorbent assay
Female
fluorescent antibody technique
heifers
IFAT
Immunoblot
immunoblotting
Infectious Disease Transmission, Vertical - veterinary
Neospora - immunology
Neospora caninum
Neosporosis
New Zealand
Outbreak
Predictive Value of Tests
Pregnancy
Pregnancy Complications, Parasitic - epidemiology
Pregnancy Complications, Parasitic - parasitology
Pregnancy Complications, Parasitic - veterinary
protozoal infections
serodiagnosis
Serologic Tests
serological surveys
seroprevalence
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Summary:Serological methods developed to detect Neospora caninum specific bovine antibodies were compared using sera from an abortion outbreak on a dairy farm in New Zealand. These methods included four ELISAs, IFAT and immunoblot. The abortions ( n=16) on the farm were restricted to one of two groups of the herd which consisted of 194 adult dairy cows. All ELISAs, IFAT and immunoblot (applying stringent cut-off levels) revealed a statistically significant association between seropositivity and abortion among the adult cows from the afflicted group 1. The strength of this association varied between different tests, and different seroprevalences were determined for various animal groups. Among the different ELISAs, the strength of the relationship between single test results was characterised by a considerable variation in the determinants of correlation. Discrepancies in positive–negative classification between the two commercial ELISAs and one of the in-house ELISAs were due mainly to differences in the cut-off selection.
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(99)00104-6