Loading…
Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA
Sensitive detection techniques are required to study the life cycle of Neospora caninum and to diagnose infections. In this study, we describe the development of a PCR assay for N. caninum based on two successive amplification steps within a single tube. This technique, called single tube nested PCR...
Saved in:
| Published in: | International journal for parasitology 1999-10, Vol.29 (10), p.1589-1596 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c444t-4d9063c0f17bf5a4850a6516a73b6f441bcd4e96dc2895289875972f079747d13 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c444t-4d9063c0f17bf5a4850a6516a73b6f441bcd4e96dc2895289875972f079747d13 |
| container_end_page | 1596 |
| container_issue | 10 |
| container_start_page | 1589 |
| container_title | International journal for parasitology |
| container_volume | 29 |
| creator | Ellis, J.T McMillan, D Ryce, C Payne, S Atkinson, R Harper, P.A.W |
| description | Sensitive detection techniques are required to study the life cycle of
Neospora caninum and to diagnose infections. In this study, we describe the development of a PCR assay for
N. caninum based on two successive amplification steps within a single tube. This technique, called single tube nested PCR, was sensitive to a single copy of target sequence, and able to amplify parasite DNA from biological specimens such as formalin-fixed, paraffin-embedded tissues of naturally infected dogs and cattle. An internal standard (or PCR MIMIC) is also described. This assay should prove useful in the study of the biology of
N. caninum. |
| doi_str_mv | 10.1016/S0020-7519(99)00144-7 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69369453</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0020751999001447</els_id><sourcerecordid>69369453</sourcerecordid><originalsourceid>FETCH-LOGICAL-c444t-4d9063c0f17bf5a4850a6516a73b6f441bcd4e96dc2895289875972f079747d13</originalsourceid><addsrcrecordid>eNqFkc1u1TAQhS0EopfCI4C8QrAI2MnYjleoavmTqrIA1pZjT6hRYgc7qXTfHrepELsuRiONvjOjOYeQl5y944zL998Za1mjBNdvtH7LGAdo1CNy4L3SDeOdeEwO_5AT8qyU3xUSHcBTcsKZZD2APJD5Am9wSsuMcaVppJaWEH9NSNdtQBqxrOjpkqbjjNkWpO7ahkgzWreGFKktxR7pmDJdr5F6XHGf101XmMqSsqXOxhC3mV5cnT0nT0Y7FXxx30_Jz08ff5x_aS6_ff56fnbZOABYG_Cayc6xkathFBZ6wawUXFrVDXIE4IPzgFp61_Za1OqV0KodmdIKlOfdKXm9711y-rPVJ8wcisNpshHTVozUndQgugdBrqDVrZQVFDvociol42iWHGabj4YzcxuIuQvE3LpttDZ3gRhVda_uD2zDjP4_1Z5ABT7sAFY_bgJmU1zA6NCHXM00PoUHTvwF5dWaGw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17429266</pqid></control><display><type>article</type><title>Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA</title><source>Elsevier</source><creator>Ellis, J.T ; McMillan, D ; Ryce, C ; Payne, S ; Atkinson, R ; Harper, P.A.W</creator><creatorcontrib>Ellis, J.T ; McMillan, D ; Ryce, C ; Payne, S ; Atkinson, R ; Harper, P.A.W</creatorcontrib><description>Sensitive detection techniques are required to study the life cycle of
Neospora caninum and to diagnose infections. In this study, we describe the development of a PCR assay for
N. caninum based on two successive amplification steps within a single tube. This technique, called single tube nested PCR, was sensitive to a single copy of target sequence, and able to amplify parasite DNA from biological specimens such as formalin-fixed, paraffin-embedded tissues of naturally infected dogs and cattle. An internal standard (or PCR MIMIC) is also described. This assay should prove useful in the study of the biology of
N. caninum.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/S0020-7519(99)00144-7</identifier><identifier>PMID: 10608446</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Abortion, Veterinary - parasitology ; Animals ; Brain - embryology ; Brain - parasitology ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - parasitology ; Coccidiosis - diagnosis ; Coccidiosis - parasitology ; Coccidiosis - veterinary ; DNA, Protozoan - analysis ; Dog Diseases - diagnosis ; Dog Diseases - parasitology ; Dogs ; Female ; Formaldehyde ; Mice ; Mice, Inbred BALB C ; Neospora - genetics ; Neospora - isolation & purification ; Neospora caninum ; Paraffin Embedding ; PCR MIMIC ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Pregnancy ; Sensitivity and Specificity ; Tissue Fixation</subject><ispartof>International journal for parasitology, 1999-10, Vol.29 (10), p.1589-1596</ispartof><rights>1999 Australian Society for Parasitology Inc</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-4d9063c0f17bf5a4850a6516a73b6f441bcd4e96dc2895289875972f079747d13</citedby><cites>FETCH-LOGICAL-c444t-4d9063c0f17bf5a4850a6516a73b6f441bcd4e96dc2895289875972f079747d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10608446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ellis, J.T</creatorcontrib><creatorcontrib>McMillan, D</creatorcontrib><creatorcontrib>Ryce, C</creatorcontrib><creatorcontrib>Payne, S</creatorcontrib><creatorcontrib>Atkinson, R</creatorcontrib><creatorcontrib>Harper, P.A.W</creatorcontrib><title>Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Sensitive detection techniques are required to study the life cycle of
Neospora caninum and to diagnose infections. In this study, we describe the development of a PCR assay for
N. caninum based on two successive amplification steps within a single tube. This technique, called single tube nested PCR, was sensitive to a single copy of target sequence, and able to amplify parasite DNA from biological specimens such as formalin-fixed, paraffin-embedded tissues of naturally infected dogs and cattle. An internal standard (or PCR MIMIC) is also described. This assay should prove useful in the study of the biology of
N. caninum.</description><subject>Abortion, Veterinary - parasitology</subject><subject>Animals</subject><subject>Brain - embryology</subject><subject>Brain - parasitology</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - parasitology</subject><subject>Coccidiosis - diagnosis</subject><subject>Coccidiosis - parasitology</subject><subject>Coccidiosis - veterinary</subject><subject>DNA, Protozoan - analysis</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>Female</subject><subject>Formaldehyde</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neospora - genetics</subject><subject>Neospora - isolation & purification</subject><subject>Neospora caninum</subject><subject>Paraffin Embedding</subject><subject>PCR MIMIC</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pregnancy</subject><subject>Sensitivity and Specificity</subject><subject>Tissue Fixation</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1TAQhS0EopfCI4C8QrAI2MnYjleoavmTqrIA1pZjT6hRYgc7qXTfHrepELsuRiONvjOjOYeQl5y944zL998Za1mjBNdvtH7LGAdo1CNy4L3SDeOdeEwO_5AT8qyU3xUSHcBTcsKZZD2APJD5Am9wSsuMcaVppJaWEH9NSNdtQBqxrOjpkqbjjNkWpO7ahkgzWreGFKktxR7pmDJdr5F6XHGf101XmMqSsqXOxhC3mV5cnT0nT0Y7FXxx30_Jz08ff5x_aS6_ff56fnbZOABYG_Cayc6xkathFBZ6wawUXFrVDXIE4IPzgFp61_Za1OqV0KodmdIKlOfdKXm9711y-rPVJ8wcisNpshHTVozUndQgugdBrqDVrZQVFDvociol42iWHGabj4YzcxuIuQvE3LpttDZ3gRhVda_uD2zDjP4_1Z5ABT7sAFY_bgJmU1zA6NCHXM00PoUHTvwF5dWaGw</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Ellis, J.T</creator><creator>McMillan, D</creator><creator>Ryce, C</creator><creator>Payne, S</creator><creator>Atkinson, R</creator><creator>Harper, P.A.W</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA</title><author>Ellis, J.T ; McMillan, D ; Ryce, C ; Payne, S ; Atkinson, R ; Harper, P.A.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-4d9063c0f17bf5a4850a6516a73b6f441bcd4e96dc2895289875972f079747d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Abortion, Veterinary - parasitology</topic><topic>Animals</topic><topic>Brain - embryology</topic><topic>Brain - parasitology</topic><topic>Cattle</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cattle Diseases - parasitology</topic><topic>Coccidiosis - diagnosis</topic><topic>Coccidiosis - parasitology</topic><topic>Coccidiosis - veterinary</topic><topic>DNA, Protozoan - analysis</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - parasitology</topic><topic>Dogs</topic><topic>Female</topic><topic>Formaldehyde</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neospora - genetics</topic><topic>Neospora - isolation & purification</topic><topic>Neospora caninum</topic><topic>Paraffin Embedding</topic><topic>PCR MIMIC</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Pregnancy</topic><topic>Sensitivity and Specificity</topic><topic>Tissue Fixation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ellis, J.T</creatorcontrib><creatorcontrib>McMillan, D</creatorcontrib><creatorcontrib>Ryce, C</creatorcontrib><creatorcontrib>Payne, S</creatorcontrib><creatorcontrib>Atkinson, R</creatorcontrib><creatorcontrib>Harper, P.A.W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ellis, J.T</au><au>McMillan, D</au><au>Ryce, C</au><au>Payne, S</au><au>Atkinson, R</au><au>Harper, P.A.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>29</volume><issue>10</issue><spage>1589</spage><epage>1596</epage><pages>1589-1596</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><abstract>Sensitive detection techniques are required to study the life cycle of
Neospora caninum and to diagnose infections. In this study, we describe the development of a PCR assay for
N. caninum based on two successive amplification steps within a single tube. This technique, called single tube nested PCR, was sensitive to a single copy of target sequence, and able to amplify parasite DNA from biological specimens such as formalin-fixed, paraffin-embedded tissues of naturally infected dogs and cattle. An internal standard (or PCR MIMIC) is also described. This assay should prove useful in the study of the biology of
N. caninum.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>10608446</pmid><doi>10.1016/S0020-7519(99)00144-7</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-7519 |
| ispartof | International journal for parasitology, 1999-10, Vol.29 (10), p.1589-1596 |
| issn | 0020-7519 1879-0135 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69369453 |
| source | Elsevier |
| subjects | Abortion, Veterinary - parasitology Animals Brain - embryology Brain - parasitology Cattle Cattle Diseases - diagnosis Cattle Diseases - parasitology Coccidiosis - diagnosis Coccidiosis - parasitology Coccidiosis - veterinary DNA, Protozoan - analysis Dog Diseases - diagnosis Dog Diseases - parasitology Dogs Female Formaldehyde Mice Mice, Inbred BALB C Neospora - genetics Neospora - isolation & purification Neospora caninum Paraffin Embedding PCR MIMIC polymerase chain reaction Polymerase Chain Reaction - methods Pregnancy Sensitivity and Specificity Tissue Fixation |
| title | Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T16%3A03%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20single%20tube%20nested%20polymerase%20chain%20reaction%20assay%20for%20the%20detection%20of%20Neospora%20caninum%20DNA&rft.jtitle=International%20journal%20for%20parasitology&rft.au=Ellis,%20J.T&rft.date=1999-10-01&rft.volume=29&rft.issue=10&rft.spage=1589&rft.epage=1596&rft.pages=1589-1596&rft.issn=0020-7519&rft.eissn=1879-0135&rft_id=info:doi/10.1016/S0020-7519(99)00144-7&rft_dat=%3Cproquest_cross%3E69369453%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c444t-4d9063c0f17bf5a4850a6516a73b6f441bcd4e96dc2895289875972f079747d13%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17429266&rft_id=info:pmid/10608446&rfr_iscdi=true |