Bovine Leukemia Virus Gag Particle Assembly in Insect Cells: Formation of Chimeric Particles by Domain-Switched Leukemia/Lentivirus Gag Polyprotein

• Published in: Virology (New York, N.Y.) N.Y.), 1999-12, Vol.265 (2), p.308-318
• Main Authors: Kakker, Naresh K., Mikhailov, Michael V., Nermut, Milan V., Burny, Arsene, Roy, Polly
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Animals; Bovine leukemia virus; Capsid - genetics; Capsid - metabolism; Cattle; Cell Line; gag gene; Gag protein; Gene Products, gag - genetics; Gene Products, gag - metabolism; Human T-lymphotropic virus 1 - genetics; Humans; Leukemia Virus, Bovine - genetics; Leukemia Virus, Bovine - physiology; Leukemia Virus, Bovine - ultrastructure; Myristic Acids - metabolism; Nucleocapsid Proteins - genetics; Nucleocapsid Proteins - metabolism; Polyproteins - genetics; Polyproteins - metabolism; Simian Immunodeficiency Virus - genetics; Spodoptera - cytology; Viral Matrix Proteins - genetics; Viral Matrix Proteins - metabolism; Virion - ultrastructure; Virus Assembly
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1006/viro.1999.0007

A key stage in the life cycle of C-type retroviruses is the assembly of Gag precursor protein at the plasma membrane of infected cells. Here we report the assembly of bovine leukemia virus (BLV) gag gene product into virus-like particles (VLPs) using the baculovirus expression system. Expression of BLV Pr44Gag resulted in the assembly and release of VLPs, thereby confirming the ability of retroviral Gag polyprotein to assemble and bud from insect cells. Efficient particle formation required a myristoylation signal at the N-terminus of BLV Pr44Gag. Recombinant baculoviruses expressing matrix (MA) or capsid–nucleocapsid (CA–NC) proteins of BLV were generated but neither of these domains was capable of assembling into particulate structures. To assess the compatibility of Gag domains between leukemia and lentivirus groups three different recombinant chimeras each expressing MA of one virus (e.g., simian immunodeficiency or BLV) and CA–NC of another (e.g., BLV or human T-cell leukemia virus type-I) were constructed. Each of the chimeric proteins assembled efficiently and budded as VLPs, suggesting that the MA and CA domains of these two evolutionary divergent retrovirus groups can be functionally exchanged without perturbation of Gag VLP formation. The lenti-leukemia chimeric Gag approach has potential for studying protein–protein interactions in other retroviruses.