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Detection of a macrophage-specific antigen and the production of interferon gamma in chickens infected with Newcastle disease virus
Formalin-fixed, paraffin-embedded spleen and intestinal tissues were harvested at 2 days postinfection from 4-wk-old white rock chickens infected with five different strains of Newcastle disease virus (NDV). These tissues were examined for the presence of macrophage antigen expression, virus replica...
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| Published in: | Avian diseases 1999-10, Vol.43 (4), p.696-703 |
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| Language: | English |
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| container_end_page | 703 |
| container_issue | 4 |
| container_start_page | 696 |
| container_title | Avian diseases |
| container_volume | 43 |
| creator | Brown, C King, D.J Seal, B |
| description | Formalin-fixed, paraffin-embedded spleen and intestinal tissues were harvested at 2 days postinfection from 4-wk-old white rock chickens infected with five different strains of Newcastle disease virus (NDV). These tissues were examined for the presence of macrophage antigen expression, virus replication, and interferon gamma (IFNgamma) production. The five strains represented all three NDV pathotypes. Viral replication and IFNgamma, as determined by riboprobe in situ hybridization, were detected only in those chickens infected with velogenic viscerotropic NDV (VVNDV) strains. Macrophage antigen expression, an indicator of macrophage activation, was determined by immunohistochemistry with a macrophage-specific antibody, CVI-ChNL-68.1. Presence of macrophage antigen was most prominent in VVNDV-infected chickens. The distribution of this antigen within tissues was far more diffuse than the staining for viral mRNA. The presence of IFNgamma mRNA was detected in the spleen and intestinal lymphoid tissue of VVNDV-infected chickens. There was also increased macrophage antigen expression in the mesogen-infected birds, but it was less dramatic than in tissues from VVNDV-infected chickens. One of two lentogen-infected birds had evidence of increased macrophage antigen expression only in the spleen. |
| doi_str_mv | 10.2307/1592739 |
| format | article |
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These tissues were examined for the presence of macrophage antigen expression, virus replication, and interferon gamma (IFNgamma) production. The five strains represented all three NDV pathotypes. Viral replication and IFNgamma, as determined by riboprobe in situ hybridization, were detected only in those chickens infected with velogenic viscerotropic NDV (VVNDV) strains. Macrophage antigen expression, an indicator of macrophage activation, was determined by immunohistochemistry with a macrophage-specific antibody, CVI-ChNL-68.1. Presence of macrophage antigen was most prominent in VVNDV-infected chickens. The distribution of this antigen within tissues was far more diffuse than the staining for viral mRNA. The presence of IFNgamma mRNA was detected in the spleen and intestinal lymphoid tissue of VVNDV-infected chickens. There was also increased macrophage antigen expression in the mesogen-infected birds, but it was less dramatic than in tissues from VVNDV-infected chickens. One of two lentogen-infected birds had evidence of increased macrophage antigen expression only in the spleen.</description><identifier>ISSN: 0005-2086</identifier><identifier>EISSN: 1938-4351</identifier><identifier>DOI: 10.2307/1592739</identifier><identifier>PMID: 10611986</identifier><language>eng</language><publisher>United States: American Association of Avian Pathologists, Inc</publisher><subject>Animals ; Antigens ; California ; Cecum - immunology ; Cecum - virology ; Chickens ; Disease Outbreaks - veterinary ; Gene Expression Regulation - immunology ; In Situ Hybridization ; Interferon-gamma - biosynthesis ; Interferon-gamma - genetics ; Interferons ; Intestine, Small - immunology ; Intestine, Small - virology ; Laboratory staining techniques ; Macrophage Activation ; Macrophages ; Macrophages - immunology ; Messenger RNA ; Newcastle Disease - epidemiology ; Newcastle Disease - immunology ; Newcastle disease virus ; Newcastle disease virus - isolation & purification ; Newcastle disease virus - physiology ; pathotypes ; RNA, Messenger - genetics ; Spleen ; Spleen - immunology ; Spleen - virology ; Transcription, Genetic ; virus replication ; Virus Replication - physiology ; Viruses</subject><ispartof>Avian diseases, 1999-10, Vol.43 (4), p.696-703</ispartof><rights>Copyright 1999 The American Association of Avian Pathologists, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-e253cfc8fe78144cd0ca370df42d8bc7bb957f9ce18b2ba664748dd53812e7d63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1592739$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1592739$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,58213,58446</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10611986$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, C</creatorcontrib><creatorcontrib>King, D.J</creatorcontrib><creatorcontrib>Seal, B</creatorcontrib><title>Detection of a macrophage-specific antigen and the production of interferon gamma in chickens infected with Newcastle disease virus</title><title>Avian diseases</title><addtitle>Avian Dis</addtitle><description>Formalin-fixed, paraffin-embedded spleen and intestinal tissues were harvested at 2 days postinfection from 4-wk-old white rock chickens infected with five different strains of Newcastle disease virus (NDV). These tissues were examined for the presence of macrophage antigen expression, virus replication, and interferon gamma (IFNgamma) production. The five strains represented all three NDV pathotypes. Viral replication and IFNgamma, as determined by riboprobe in situ hybridization, were detected only in those chickens infected with velogenic viscerotropic NDV (VVNDV) strains. Macrophage antigen expression, an indicator of macrophage activation, was determined by immunohistochemistry with a macrophage-specific antibody, CVI-ChNL-68.1. Presence of macrophage antigen was most prominent in VVNDV-infected chickens. The distribution of this antigen within tissues was far more diffuse than the staining for viral mRNA. The presence of IFNgamma mRNA was detected in the spleen and intestinal lymphoid tissue of VVNDV-infected chickens. There was also increased macrophage antigen expression in the mesogen-infected birds, but it was less dramatic than in tissues from VVNDV-infected chickens. One of two lentogen-infected birds had evidence of increased macrophage antigen expression only in the spleen.</description><subject>Animals</subject><subject>Antigens</subject><subject>California</subject><subject>Cecum - immunology</subject><subject>Cecum - virology</subject><subject>Chickens</subject><subject>Disease Outbreaks - veterinary</subject><subject>Gene Expression Regulation - immunology</subject><subject>In Situ Hybridization</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - genetics</subject><subject>Interferons</subject><subject>Intestine, Small - immunology</subject><subject>Intestine, Small - virology</subject><subject>Laboratory staining techniques</subject><subject>Macrophage Activation</subject><subject>Macrophages</subject><subject>Macrophages - immunology</subject><subject>Messenger RNA</subject><subject>Newcastle Disease - epidemiology</subject><subject>Newcastle Disease - immunology</subject><subject>Newcastle disease virus</subject><subject>Newcastle disease virus - isolation & purification</subject><subject>Newcastle disease virus - physiology</subject><subject>pathotypes</subject><subject>RNA, Messenger - genetics</subject><subject>Spleen</subject><subject>Spleen - immunology</subject><subject>Spleen - virology</subject><subject>Transcription, Genetic</subject><subject>virus replication</subject><subject>Virus Replication - physiology</subject><subject>Viruses</subject><issn>0005-2086</issn><issn>1938-4351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNp9kE1P3DAQhq2qCBaK-g9aHxA9hY7tJLaPiH5KCA6Uc-TY413TTbK1nSLO_HFcZVX11NN86JlXmoeQtwwuuAD5kTWaS6FfkRXTQlW1aNhrsgKApuKg2iNynNIDAJO6hUNyxKBlTKt2RZ4_YUabwzTSyVNDB2PjtNuYNVZphzb4YKkZc1jjWKqjeYN0Fyc3_70JY8boMZZpbYbBlAW1m2B_4phK70s6OvoY8obe4KM1KW-RupDQJKS_Q5zTG3LgzTbh6b6ekPsvn39cfauub79-v7q8rqwQkCvkjbDeKo9Ssbq2DqwREpyvuVO9lX2vG-m1RaZ63pu2rWWtnGuEYhyla8UJOV9yywO_Zky5G0KyuN2aEac5da0WshZaFvDDAhYXKUX03S6GwcSnjkH3x3e3913Id_vIuR_Q_cMtggtwtgAPKU_xPznvF8ybqTPrGFJ3f8eBCeCai1qAeAHWxJEa</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Brown, C</creator><creator>King, D.J</creator><creator>Seal, B</creator><general>American Association of Avian Pathologists, Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Detection of a macrophage-specific antigen and the production of interferon gamma in chickens infected with Newcastle disease virus</title><author>Brown, C ; King, D.J ; Seal, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-e253cfc8fe78144cd0ca370df42d8bc7bb957f9ce18b2ba664748dd53812e7d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>California</topic><topic>Cecum - immunology</topic><topic>Cecum - virology</topic><topic>Chickens</topic><topic>Disease Outbreaks - veterinary</topic><topic>Gene Expression Regulation - immunology</topic><topic>In Situ Hybridization</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-gamma - genetics</topic><topic>Interferons</topic><topic>Intestine, Small - immunology</topic><topic>Intestine, Small - virology</topic><topic>Laboratory staining techniques</topic><topic>Macrophage Activation</topic><topic>Macrophages</topic><topic>Macrophages - immunology</topic><topic>Messenger RNA</topic><topic>Newcastle Disease - epidemiology</topic><topic>Newcastle Disease - immunology</topic><topic>Newcastle disease virus</topic><topic>Newcastle disease virus - isolation & purification</topic><topic>Newcastle disease virus - physiology</topic><topic>pathotypes</topic><topic>RNA, Messenger - genetics</topic><topic>Spleen</topic><topic>Spleen - immunology</topic><topic>Spleen - virology</topic><topic>Transcription, Genetic</topic><topic>virus replication</topic><topic>Virus Replication - physiology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, C</creatorcontrib><creatorcontrib>King, D.J</creatorcontrib><creatorcontrib>Seal, B</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Avian diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, C</au><au>King, D.J</au><au>Seal, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of a macrophage-specific antigen and the production of interferon gamma in chickens infected with Newcastle disease virus</atitle><jtitle>Avian diseases</jtitle><addtitle>Avian Dis</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>43</volume><issue>4</issue><spage>696</spage><epage>703</epage><pages>696-703</pages><issn>0005-2086</issn><eissn>1938-4351</eissn><abstract>Formalin-fixed, paraffin-embedded spleen and intestinal tissues were harvested at 2 days postinfection from 4-wk-old white rock chickens infected with five different strains of Newcastle disease virus (NDV). These tissues were examined for the presence of macrophage antigen expression, virus replication, and interferon gamma (IFNgamma) production. The five strains represented all three NDV pathotypes. Viral replication and IFNgamma, as determined by riboprobe in situ hybridization, were detected only in those chickens infected with velogenic viscerotropic NDV (VVNDV) strains. Macrophage antigen expression, an indicator of macrophage activation, was determined by immunohistochemistry with a macrophage-specific antibody, CVI-ChNL-68.1. Presence of macrophage antigen was most prominent in VVNDV-infected chickens. The distribution of this antigen within tissues was far more diffuse than the staining for viral mRNA. The presence of IFNgamma mRNA was detected in the spleen and intestinal lymphoid tissue of VVNDV-infected chickens. There was also increased macrophage antigen expression in the mesogen-infected birds, but it was less dramatic than in tissues from VVNDV-infected chickens. One of two lentogen-infected birds had evidence of increased macrophage antigen expression only in the spleen.</abstract><cop>United States</cop><pub>American Association of Avian Pathologists, Inc</pub><pmid>10611986</pmid><doi>10.2307/1592739</doi><tpages>8</tpages></addata></record> |
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| ispartof | Avian diseases, 1999-10, Vol.43 (4), p.696-703 |
| issn | 0005-2086 1938-4351 |
| language | eng |
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| source | JSTOR |
| subjects | Animals Antigens California Cecum - immunology Cecum - virology Chickens Disease Outbreaks - veterinary Gene Expression Regulation - immunology In Situ Hybridization Interferon-gamma - biosynthesis Interferon-gamma - genetics Interferons Intestine, Small - immunology Intestine, Small - virology Laboratory staining techniques Macrophage Activation Macrophages Macrophages - immunology Messenger RNA Newcastle Disease - epidemiology Newcastle Disease - immunology Newcastle disease virus Newcastle disease virus - isolation & purification Newcastle disease virus - physiology pathotypes RNA, Messenger - genetics Spleen Spleen - immunology Spleen - virology Transcription, Genetic virus replication Virus Replication - physiology Viruses |
| title | Detection of a macrophage-specific antigen and the production of interferon gamma in chickens infected with Newcastle disease virus |
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