Label-free biosensor by protein grating coupler on planar optical waveguides

We report a diffraction-based, label-free biosensor on the surface of a planar optical waveguide. By selectively immobilizing proteins on an optical waveguide, the proteins can form the ridge/valley of a latent image optical grating. Binding events with patterned surface proteins dynamically increas...

Full description

Saved in:
Bibliographic Details
Published in:Optics letters 2008-08, Vol.33 (15), p.1735-1737
Main Authors: ZHIAN LAI, YULI WANG, ALLBRITTON, Nancy, LI, G.-P, BACHMAN, Mark
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Cattle
Circuit properties
Electric, optical and optoelectronic circuits
Electronics
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Immunoglobulin G - metabolism
Integrated optics. Optical fibers and wave guides
Lasers
Methods. Procedures. Technologies
Optical and optoelectronic circuits
Optics and Photonics - instrumentation
Protein Binding
Refractometry - instrumentation
Refractometry - methods
Serum Albumin, Bovine - metabolism
Surface Properties
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report a diffraction-based, label-free biosensor on the surface of a planar optical waveguide. By selectively immobilizing proteins on an optical waveguide, the proteins can form the ridge/valley of a latent image optical grating. Binding events with patterned surface proteins dynamically increase grating height and cause an increase in light coupling out of the waveguide. We demonstrate the efficacy of this strategy for protein detection and present the design and fabrication of a device.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.33.001735