Galectin-1 in cartilage: Expression, influence on chondrocyte growth and interaction with ECM components

Galectin-1 is a 14 kDa beta-galactoside binding protein, capable of forming lattice-like structures with glycans of cellular glycoconjugates and inducing intracellular signaling. The expression of Galectin-1 in porcine cartilage is described in this work for the first time. Immunocytochemical method...

Full description

Saved in:
Bibliographic Details
Published in:Matrix biology 2008-07, Vol.27 (6), p.513-525
Main Authors: Marsich, Eleonora, Mozetic, Pamela, Ortolani, Fulvia, Contin, Magali, Marchini, Maurizio, Vetere, Amedeo, Pacor, Sabrina, Semeraro, Sabrina, Vittur, Franco, Paoletti, Sergio
Format: Article
Language:English
Subjects:
Animals
Cartilage
Cartilage - cytology
Cartilage - metabolism
Cell Adhesion - physiology
Cell Cycle - physiology
Cell Proliferation
Chondrocyte growth
Chondrocytes - cytology
Chondrocytes - physiology
ECM
Extracellular Matrix - chemistry
Extracellular Matrix - metabolism
Galectin 1 - genetics
Galectin 1 - metabolism
Galectin-1
Swine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Galectin-1 is a 14 kDa beta-galactoside binding protein, capable of forming lattice-like structures with glycans of cellular glycoconjugates and inducing intracellular signaling. The expression of Galectin-1 in porcine cartilage is described in this work for the first time. Immunocytochemical methods revealed distinct distribution patterns for both articular and growth plate cartilage. In articular cartilage, the highest reactivity for Galectin-1 was found in all chondrocytes at the superficial zone and in most of those at the lower layer of the middle zone. In the growth plate, marked reactivity was seen in chondrocytes at the proliferative zone and reached a maximum level for the column-forming cells at the hypertrophic zone. In addition, different Galectin-1 distribution patterns were observed at the subcellular level. With regards to the metabolic effects of Galectin-1, the results in vitro seem to indicate an inhibitory effect of Galectin-1 on articular chondrocyte anabolism ( i.e. inhibition of cell proliferation and anabolic gene expression) and a stimulation of catabolic processes ( i.e. induction of matrix degradation and hypertrophy marker expression). These data represent a starting point for the understanding the molecular mechanisms underlining ECM–Galectin-1 interaction and the subsequent signaling–cell transduction processes involving cartilage formation and maturation.
ISSN:0945-053X
1569-1802
DOI:10.1016/j.matbio.2008.04.003