Modulation of chlorogenic acid biosynthesis in Solanum lycopersicum; consequences for phenolic accumulation and UV-tolerance

• Published in: Phytochemistry (Oxford) 2008-08, Vol.69 (11), p.2149-2156
• Main Authors: Clé, Carla, Hill, Lionel M., Niggeweg, Ricarda, Martin, Cathie R., Guisez, Yves, Prinsen, Els, Jansen, Marcel A.K.
• Format: Article
• Language: English
• Subjects: alkyl (aryl) transferases; Biological and medical sciences; Chemical constitution; Chlorogenic acid; Chlorogenic Acid - chemistry; Chlorogenic Acid - metabolism; Chlorophyll - chemistry; Chlorophyll - metabolism; Fundamental and applied biological sciences. Psychology; gene expression regulation; gene overexpression; gene silencing; hydroxycinnamoyl CoA quinate transferase; Lycopersicon esculentum - chemistry; Lycopersicon esculentum - metabolism; Mass Spectrometry; Metabolism; Metabolism. Physicochemical requirements; phenolic compounds; Phenols - metabolism; Phenylpropanoid pathway; phenylpropanoids; Plant Extracts - chemistry; Plant physiology and development; Rutin; Solanum - metabolism; Solanum - radiation effects; Solanum lycopersicum; Solanum lycopersicum var. lycopersicum; Solubility; Spectrum Analysis; Tomato; tomatoes; transgenes; transgenic plants; UV-B radiation
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2008.04.024

Hydroxycinnamoyl CoA quinate transferase (HQT) mediates chlorogenic acid biosynthesis in tomato (Solanum lycopersicum). Our study revealed that increased chlorogenic acid accumulation was associated with UV-protection in transgenics with altered HQT activity. However, manipulation of HQT activity also resulted in more complex alterations in the profiles of phenolics. Our data suggests the existence of regulatory mechanisms that direct the flow of phenolic precursors in response to both metabolic parameters and environmental conditions. Chlorogenic acid (CGA) is one of the most abundant phenolic compounds in tomato (Solanum lycopersicum). Hydroxycinnamoyl CoA quinate transferase (HQT) is the key enzyme catalysing CGA biosynthesis in tomato. We have studied the relationship between phenolic accumulation and UV-susceptibility in transgenic tomato plants with altered HQT expression. Overall, increased CGA accumulation was associated with increased UV-protection. However, the genetic manipulation of HQT expression also resulted in more complex alterations in the profiles of phenolics. Levels of rutin were relatively high in both HQT gene-silenced and HQT-overexpressing plants raised in plant growth tunnels. This suggests plasticity in the flux along different branches of phenylpropanoid metabolism and the existence of regulatory mechanisms that direct the flow of phenolic precursors in response to both metabolic parameters and environmental conditions. These changes in composition of the phenolic pool affected the relative levels of UV-tolerance. We conclude that the capability of the phenolic compounds to protect against potentially harmful UV radiation is determined both by the total levels of phenolics that accumulate in leaves as well as by the specific composition of the phenolic profile.