Parental Expression of the Chromodomain Protein Pdd1p Is Required for Completion of Programmed DNA Elimination and Nuclear Differentiation

Thousands of DNA elimination events occur during somatic differentiation of many ciliated protozoa. In Tetrahymena, the eliminated DNA aggregates into submacronuclear structures containing the protein Pdd1p, a member of the chromodomain family. We disrupted somatic copies of PDD1, eliminating parent...

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Bibliographic Details
Published in:Molecular cell 1999-11, Vol.4 (5), p.865-872
Main Authors: Coyne, Robert S, Nikiforov, Mikhail A, Smothers, James F, Allis, C.David, Yao, Meng-Chao
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cell Nucleus - genetics
Cell Nucleus - metabolism
Chromatin - chemistry
Chromatin - genetics
Chromatin - metabolism
Chromosome Breakage - genetics
Chromosome Segregation - genetics
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
DNA, Protozoan - metabolism
Gene Deletion
Gene Duplication
Gene Expression
Genes, Lethal - genetics
Genome, Protozoan
Micronucleus, Germline - genetics
Micronucleus, Germline - metabolism
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
PDD1 gene
Pdd1 protein
Phosphoproteins - genetics
Phosphoproteins - metabolism
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Tetrahymena
Tetrahymena - cytology
Tetrahymena - genetics
Tetrahymena - growth & development
Transformation, Genetic
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Summary:Thousands of DNA elimination events occur during somatic differentiation of many ciliated protozoa. In Tetrahymena, the eliminated DNA aggregates into submacronuclear structures containing the protein Pdd1p, a member of the chromodomain family. We disrupted somatic copies of PDD1, eliminating parental expression of the gene early in the sexual phase of the life cycle. Even though zygotic expression, from the undisrupted germline PDD1 copy, is activated before DNA elimination normally occurs, the somatic knockout cells suffer defects in DNA elimination, genome endoduplication, and nuclear resorption, and eventually die, demonstrating that PDD1 is essential and suggesting Pdd1p is directly involved in establishing a chromatin structure required for DNA elimination.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(00)80396-2