Dual-Color STED Microscopy at 30-nm Focal-Plane Resolution

Stimulated emission depletion (STED) fluorescence microscopy images protein distribution in cells in two‐color channels at a resolution of ≈30 nm in the focal plane (see image), corresponding to a seven‐to eightfold improvement over confocal microscopy resolution.

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2008-08, Vol.4 (8), p.1095-1100
Main Authors: Meyer, Lars, Wildanger, Dominik, Medda, Rebecca, Punge, Annedore, Rizzoli, Silvio O., Donnert, Gerald, Hell, Stefan W.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Cells, Cultured
fluorescence spectroscopy
Fluorescent Dyes
imaging
Membrane Glycoproteins - analysis
Microscopy, Confocal
Microscopy, Fluorescence - methods
optics
Rats
Spectrometry, Fluorescence
STED
Syntaxin 1 - analysis
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Description
Summary:Stimulated emission depletion (STED) fluorescence microscopy images protein distribution in cells in two‐color channels at a resolution of ≈30 nm in the focal plane (see image), corresponding to a seven‐to eightfold improvement over confocal microscopy resolution.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200800055