A transposon, Ping, is integrated into intron 4 of the DROOPING LEAF gene of rice [Oryza sativa], weakly reducing its expression and causing a mild drooping leaf phenotype

The YABBY gene DROOPING LEAF (DL) regulates midrib formation in the leaves and carpel specification in the flowers of rice (Oryza sativa L). We found a new dl allele (dl-5) that caused a mild phenotype in the descendants of a mutable line, IM294. In plants homozygous for this allele, midrib structur...

Full description

Saved in:
Bibliographic Details
Published in:Plant and cell physiology 2008-08, Vol.49 (8), p.1176-1184
Main Authors: Ohmori, Y.(Tokyo Univ. (Japan)), Abiko, M, Horibata, A, Hirano, H
Format: Article
Language:English
Subjects:
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
ARN
DNA Transposable Elements - genetics
DROOPING LEAF (DL)
FEUILLE
GENE
GENES
Genes, Plant
HIBRIDACION
HOJAS
HYBRIDATION
HYBRIDIZATION
INBRED LINES
Introns - genetics
Leaf Development
LEAVES
LIGNEE CONSANGUINE
LINEAS CONSANGUINEAS
Midrib
MUTANT
MUTANTES
MUTANTS
Mutation
NUCLEOTIDE SEQUENCE
Oryza - anatomy & histology
Oryza - genetics
ORYZA SATIVA
Oryza sativa (rice)
PCR
Ping Transposon
PLANT ANATOMY
Plant Leaves - anatomy & histology
Plant Leaves - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
RNA
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
YABBY gene
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The YABBY gene DROOPING LEAF (DL) regulates midrib formation in the leaves and carpel specification in the flowers of rice (Oryza sativa L). We found a new dl allele (dl-5) that caused a mild phenotype in the descendants of a mutable line, IM294. In plants homozygous for this allele, midrib structures were formed but their sizes were reduced. Molecular analysis revealed that a transposon, Ping, was inserted in the fourth intron of DL. Together with mPing and Pong, Ping is a member of a transposon family that was first identified as a group of active transposable elements in rice. Our finding of the Ping insertion in the DL gene is a first indication that Ping is active in planta, and that it can be transposed and integrated in a new locus. Ping seems to be still active because it was excised from intron 4 of DL at a relatively high frequency in rice calli. Real-time PCR analysis and in situ hybridization indicated that DL transcript levels were reduced in dl-5 without alterations in the spatial expression pattern of the DL gene. The reduction of DL expression may be due to inefficient splicing of the large intron caused by Ping insertion. By comparing the expression levels of DL and leaf phenotypes in the dl mutants with different severities, we confirmed our previous hypothesis that DL promotes cell proliferation in the central region of leaf primordia, and that this cell proliferation is critical for midrib formation in the mature leaves.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcn093