Identification of nucleoli in the early branching protist Giardia duodenalis

Giardia duodenalis has been described as ‘anucleolated’. In this work we analysed the subcellular distribution of several nucleolar markers in Giardia nuclei using silver and immunostaining techniques for electron and confocal laser microscopy as well as expression of epitope-tagged proteins in tran...

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Bibliographic Details
Published in:International journal for parasitology 2008-09, Vol.38 (11), p.1297-1304
Main Authors: Jiménez-García, Luis Felipe, Zavala, Guadalupe, Chávez-Munguía, Bibiana, Ramos-Godínez, María del Pilar, López-Velázquez, Gabriel, Segura-Valdez, María de Lourdes, Montañez, Cecilia, Hehl, Adrian B., Argüello-García, Raúl, Ortega-Pierres, Guadalupe
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Nucleolus - metabolism
Cell Nucleolus - ultrastructure
Chromosomal Proteins, Non-Histone - genetics
Diplomonads
Evolution, Molecular
Fundamental and applied biological sciences. Psychology
Giardia
Giardia - metabolism
Giardia - ultrastructure
Giardia duodenalis
Humans
Life cycle. Host-agent relationship. Pathogenesis
Microscopy, Confocal
Microscopy, Electron
Nucleolar organising region
Nucleolus
Nucleolus Organizer Region - metabolism
Nucleolus Organizer Region - ultrastructure
Protozoa
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Ribonucleoproteins
RNA, Ribosomal - genetics
RNA, Ribosomal - metabolism
rRNA processing
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Description
Summary:Giardia duodenalis has been described as ‘anucleolated’. In this work we analysed the subcellular distribution of several nucleolar markers in Giardia nuclei using silver and immunostaining techniques for electron and confocal laser microscopy as well as expression of epitope-tagged proteins in transgenic trophozoites. We identified anteronuclear fibrogranular structures corresponding to nucleolar organising regions with recruited ribonucleoprotein complexes, rRNA and epitope-tagged fibrillarin and rRNA-pseudouridine synthase (CBF5). Recombinant fibrillarin and CBF5 were targeted to this subcompartment. This study demonstrates the presence of nucleoli in G. duodenalis and provides a model to analyse minimal requirements for nucleolar assembly and maintenance in eukaryotic cells.
ISSN:0020-7519
1879-0135
DOI:10.1016/j.ijpara.2008.04.012