Development of dual scale scaffolds via direct polymer melt deposition and electrospinning for applications in tissue regeneration

The objective of this study was the fabrication of highly functionalized polymeric three-dimensional (3D) structures characterized by nano and microfibers for use as an extracellular matrix-like tissue engineering scaffold. A hybrid process utilizing direct polymer melt deposition (DPMD) and an elec...

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Bibliographic Details
Published in:Acta biomaterialia 2008-09, Vol.4 (5), p.1198-1207
Main Authors: Park, Suk Hee, Kim, Taek Gyoung, Kim, Hyo Chan, Yang, Dong-Yol, Park, Tae Gwan
Format: Article
Language:English
Subjects:
3D hybrid scaffolds
Animals
Biocompatible Materials - chemistry
Cattle
Cell Culture Techniques - methods
Cell Proliferation
Cells, Cultured
Chondrocytes - cytology
Chondrocytes - physiology
Direct polymer melt deposition (DPMD)
Electrochemistry - methods
Electrospinning
Guided Tissue Regeneration - instrumentation
Guided Tissue Regeneration - methods
Hot Temperature
Materials Testing
Microfiber
Nanofiber
Polyesters - chemistry
Polymers - chemistry
Rotation
Tissue Engineering - methods
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Summary:The objective of this study was the fabrication of highly functionalized polymeric three-dimensional (3D) structures characterized by nano and microfibers for use as an extracellular matrix-like tissue engineering scaffold. A hybrid process utilizing direct polymer melt deposition (DPMD) and an electrospinning method were employed to obtain the structure. Each microfibrous layer of the scaffold was built using the DPMD process in accordance with computer-aided design modeling data considering some structural points such as pore size, pore interconnectivity and fiber diameter. Between the layers of the three-dimensional structure, polycaprolactone/collagen nanofiber matrices were deposited via an electrospinning process. To evaluate the fabricated scaffolds, chondrocytes were seeded and cultured within the developed scaffolds for 10 days, and the levels of cell adhesion and proliferation were monitored. The results showed that the polymeric scaffolds with nanofiber matrices fabricated using the proposed hybrid process provided favorable conditions for cell adhesion and proliferation. These conditions can be attributed to enhanced cytocompatibility of the scaffold due to surficial nanotopography in the scaffold, chemical composition by use of a functional biocomposite, and an enlarged inner surface of the structure for cell attachment and growth.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2008.03.019