Improved attachment of mesenchymal stem cells on super-hydrophobic TiO2 nanotubes

Self-organized layers of vertically orientated TiO(2) nanotubes providing defined diameters ranging from 15 up to 100nm were grown on titanium by anodic oxidation. These TiO(2) nanotube layers show super-hydrophilic behavior. After coating TiO(2) nanotube layers with a self-assembled monolayer (octa...

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Bibliographic Details
Published in:Acta biomaterialia 2008-09, Vol.4 (5), p.1576-1582
Main Authors: Bauer, Sebastian, Park, Jung, von der Mark, Klaus, Schmuki, Patrik
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
Cell Adhesion - physiology
Cells, Cultured
Hydrophobic and Hydrophilic Interactions
Materials Testing
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Nanotubes - chemistry
Nanotubes - ultrastructure
Rats
Rats, Wistar
Titanium - chemistry
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Summary:Self-organized layers of vertically orientated TiO(2) nanotubes providing defined diameters ranging from 15 up to 100nm were grown on titanium by anodic oxidation. These TiO(2) nanotube layers show super-hydrophilic behavior. After coating TiO(2) nanotube layers with a self-assembled monolayer (octadecylphosphonic acid) they showed a diameter-dependent wetting behavior ranging from hydrophobic (108+/-2 degrees ) up to super-hydrophobic (167+/-2 degrees ). Cell adhesion, spreading and growth of mesenchymal stem cells on the unmodified and modified nanotube layers were investigated and compared. We show that cell adhesion and proliferation are strongly affected in the super-hydrophobic range. Adsorption of extracellular matrix proteins as fibronectin, type I collagen and laminin, as well as bovine serum albumin, on the coated and uncoated surfaces showed a strong influence on wetting behavior and dependence on tube diameter.
ISSN:1742-7061
DOI:10.1016/j.actbio.2008.04.004