Iron and radical content in Mya arenaria Possible sources of NO generation

The objective of this work was to analyze oxidative metabolism in Mya arenaria. Total Fe content in M. arenaria collected in the German Wadden Sea was 1.9+/-0.7, 0.7+/-0.1 and 0.17+/-0.01 nmol/mg fresh weight (FW), in digestive glands (DG), mantle and gills, respectively. Labile Fe pool, assessed by...

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Bibliographic Details
Published in:Aquatic toxicology 2008-08, Vol.89 (2), p.122-128
Main Authors: GONZALEZ, Paula Mariela, ABELE, Doris, PUNTARULO, Susana
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Digestive System - metabolism
Ecotoxicology, biological effects of pollution
Electron Spin Resonance Spectroscopy
Free Radicals - metabolism
Fundamental and applied biological sciences. Psychology
General aspects
Iron - metabolism
Mya - enzymology
Mya - growth & development
Mya - metabolism
Nitric Oxide - biosynthesis
Nitric Oxide Synthase - metabolism
Oxidative Stress
Substrate Specificity
Thiobarbituric Acid Reactive Substances - metabolism
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Summary:The objective of this work was to analyze oxidative metabolism in Mya arenaria. Total Fe content in M. arenaria collected in the German Wadden Sea was 1.9+/-0.7, 0.7+/-0.1 and 0.17+/-0.01 nmol/mg fresh weight (FW), in digestive glands (DG), mantle and gills, respectively. Labile Fe pool, assessed by electronic paramagnetic resonance (EPR), was 146+/-10 pmol/mg FW, and by the fluorescence method employing calcein it was 118+/-9 pmol/mg FW. The lipid radical content in the DG, assessed by EPR, was 27+/-7 pmol/mg FW, and the thiobarbituric reactive substances content amounted to 57+/-8 pmol/mg FW. Ascorbyl radical (A) content, assessed by quantification of EPR signals, was 0.04+/-0.01 pmol/mg FW, and the ascorbate content (AH(-)) was 478+/-12 pmol/mg FW. The ratio A/AH(-) was (8+/-1)x10(-5)AU, suggesting a minimum oxidative stress even under physiological conditions, presumably depending on basal metabolic functions. The content of nitric oxide (NO), assessed by EPR, was 99+/-3 pmol/mg FW. The generation rate of NO by nitric oxide synthase-like activity (NOS-like) was assayed as NO production detected by EPR in the presence of l-arginine and NADPH, and was 3.16+/-0.06 pmol/(mg FW min). The data presented here document the detectable presence of highly reactive species in M. arenaria.
ISSN:0166-445X
1879-1514
DOI:10.1016/j.aquatox.2008.06.008