Murine Müller cells are progenitor cells for neuronal cells and fibrous tissue cells

Mammalian Müller cells have been reported to possess retinal progenitor cell properties and generate new neurons after injury. This study investigates murine Müller cells under in vitro conditions for their capability of dedifferentiation into retinal progenitor cells. Müller cells were isolated fro...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-09, Vol.374 (2), p.187-191
Main Authors: Florian, Christian, Langmann, Thomas, Weber, Bernhard H.F., Morsczeck, Christian
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cells, Cultured
Cellular Senescence
Culture Media - metabolism
Dedifferentiation
Gliosis
Laminin - metabolism
Mice
Mice, Inbred Strains
Neurons - cytology
Ornithine - metabolism
Proliferation
Retina - cytology
Retinal regeneration
Stem cells
Stem Cells - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mammalian Müller cells have been reported to possess retinal progenitor cell properties and generate new neurons after injury. This study investigates murine Müller cells under in vitro conditions for their capability of dedifferentiation into retinal progenitor cells. Müller cells were isolated from mouse retina, and proliferating cells were expanded in serum-containing medium. For dedifferentiation, the cultured cells were transferred to serum-replacement medium (SRM) at different points in time after their isolation. Interestingly, early cell passages produced fibrous tissue in which extracellular matrix proteins and connective tissue markers were differentially expressed. In contrast, aged Müller cell cultures formed neurospheres in SRM that are characteristic for neuronal progenitor cells. These neurospheres differentiated into neuron-like cells after cultivation on laminin/ornithine cell culture substrate. Here, we report for the first time that murine Müller cells can be progenitors for both, fibrous tissue cells and neuronal cells, depending on the age of the cell culture.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.06.119