Gas chromatography/mass spectrometry in metabolic profiling of biological fluids

One of the objectives of metabonomics is to identify subtle changes in metabolite profiles between biological systems of different physiological or pathological states. Gas chromatography mass spectrometry (GC/MS) is a widely used analytical tool for metabolic profiling in various biofluids, such as...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-08, Vol.871 (2), p.202-211
Main Authors: Pasikanti, Kishore K., Ho, P.C., Chan, E.C.Y.
Format: Article
Language:English
Subjects:
Animals
Blood
Body Fluids - chemistry
Body Fluids - metabolism
Computational Biology - methods
Gas chromatography
Gas Chromatography-Mass Spectrometry - instrumentation
Gas Chromatography-Mass Spectrometry - methods
GC/MS
Humans
Metabolic profiling
Metabolism
Metabolomics
Metabonomics
Plasma - chemistry
Urine
Urine - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:One of the objectives of metabonomics is to identify subtle changes in metabolite profiles between biological systems of different physiological or pathological states. Gas chromatography mass spectrometry (GC/MS) is a widely used analytical tool for metabolic profiling in various biofluids, such as urine and blood due to its high sensitivity, peak resolution and reproducibility. The availability of the GC/MS electron impact (EI) spectral library further facilitates the identification of diagnostic biomarkers and aids the subsequent mechanistic elucidation of the biological or pathological variations. With the advent of new comprehensive two dimensional GC (GC × GC) coupled to time-of-flight mass spectrometry (TOFMS), it is possible to detect more than 1200 compounds in a single analytical run. In this review, we discuss the applications of GC/MS in the metabolic profiling of urine and blood, and discuss its advances in methodologies and technologies.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.04.033