Complementary mating types of Mucor circinelloides show electrophoretic karyotype heterogeneity

Electrophoretic karyotypes of ten strains of Mucor circinelloides f. lusitanicus were generated by contour-clamped homogeneous electric field (CHEF) gel-electrophoresis. Most of the strains analyzed showed polymorphisms, but a different main karyotype pattern could be correlated with each mating typ...

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Bibliographic Details
Published in:Current genetics 1999-12, Vol.36 (6), p.383-389
Main Authors: Diaz-Minguez, J.M, Lopez-Matas, M.A, Eslava, A.P
Format: Article
Language:English
Subjects:
Blotting, Southern
chromosome mapping
chromosome polymorphism
chromosomes
Chromosomes, Fungal - genetics
chs3 gene
contour-clamped homogeneous electric field gel electrophoresis
DNA
DNA probes
DNA, Ribosomal - genetics
Electrophoresis, Gel, Pulsed-Field - methods
gene location
genes
Genetic Complementation Test
Genetic Heterogeneity
Genetic Linkage
karyotyping
linkage (genetics)
mating behavior
Mucor
Mucor - chemistry
Mucor - genetics
Mucor circinelloides
mucor circinelloides f. lusitanicus
Polymorphism, Genetic
pulsed field electrophoresis
pyrF gene
Reproduction
sexual reproduction
Southern blotting
types
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Summary:Electrophoretic karyotypes of ten strains of Mucor circinelloides f. lusitanicus were generated by contour-clamped homogeneous electric field (CHEF) gel-electrophoresis. Most of the strains analyzed showed polymorphisms, but a different main karyotype pattern could be correlated with each mating type. Genome structure was further analyzed by gene assignment to the chromosome-sized DNAs. Nonradioactive hybridization techniques identified the chromosomal localization of seven cloned genes. The hybridization patterns confirmed the similarity between the mating-type (-) strains and showed some heterogeneity among the mating-type (+) strains. Linkage was found between genes pyrF and chs3 in all the strains, between the gene leuA and the rDNA in all mating-type (-) strains and ATCC 1216b (+), and between chs2 and the rDNA in CBS 969.68 (+). This is the first time that gene linkage in M. circinelloides has been reported, but in some cases the linkage relationships obtained are strain-dependent.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050513