Molecular cytogenetics of Salminus fish (Characiformes) based on 5S and 18S rRNA genes hybridization, fluorochrome staining and C-banding

Karyotype, mapping of nucleolar and 5S rRNA genes and distribution of constitutive heterochromatin supposedly AT-rich were characterized on two isolate populations of Salminus brasiliensis, the biggest characid fish, and three population of Salminus hilarii. The diploid number 2 n = 50 and the karyo...

Full description

Saved in:
Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 2008-10, Vol.39 (7), p.1036-1041
Main Authors: Souza, Issakar Lima, Santos-Silva, Ludier Kesser, Venere, Paulo César, Moreira-Filho, Orlando
Format: Article
Language:English
Subjects:
Animals
Chromomycin A 3
Chromosome Banding
Chromosomes - genetics
Constitutive heterochromatin
Cytogenetic Analysis
DA/DAPI
Fishes - classification
Fishes - genetics
Fluorescent Dyes - metabolism
In Situ Hybridization, Fluorescence
Nucleic Acid Hybridization
RNA, Ribosomal, 18S - genetics
RNA, Ribosomal, 5S - genetics
Salminus
Staining and Labeling
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Karyotype, mapping of nucleolar and 5S rRNA genes and distribution of constitutive heterochromatin supposedly AT-rich were characterized on two isolate populations of Salminus brasiliensis, the biggest characid fish, and three population of Salminus hilarii. The diploid number 2 n = 50 and the karyotype formulae (10M + 20SM + 20ST/A) were the same to Salminus species studied. The position of 18S rDNA cluster identified by FISH coincide with chromomycin A 3 labeling (CMA +) in the long arm telomeric portion of sixth pair. Subtle differences for the disposal of the 5S rRNA gene in the chromosome of the Salminus are presented. The distribution of the constitutive heterochromatins and DA/DAPI + bands are described.
ISSN:0968-4328
1878-4291
DOI:10.1016/j.micron.2007.09.007