Fas-resistance in ATL cell lines not associated with HTLV-I or FAP-1 production

• Published in: Cancer letters 1999-12, Vol.147 (1), p.215-219
• Main Authors: Yamada, Yasuaki, Sugahara, Kazuyuki, Tsuruda, Kazuto, Nohda, Kazuo, Hata, Tomoko, Maeda, Takahiro, Honda, Miyuki, Tawara, Masayuki, Hayashibara, Toshihisa, Joh, Tatsuroh, Tomonaga, Masao, Miyazaki, Yoshitsugu, Kamihira, Shimeru
• Format: Article
• Language: English
• Subjects: Adult T-cell leukemia; AIDS/HIV; Antibodies, Monoclonal - pharmacology; Apoptosis; Apoptosis - drug effects; Apoptosis - immunology; Biological and medical sciences; Blotting, Southern; Carrier Proteins - biosynthesis; Carrier Proteins - genetics; Carrier Proteins - immunology; Clone Cells; DNA Fragmentation - drug effects; DNA, Complementary - biosynthesis; Drug Resistance, Neoplasm; Fas; Fas antigen; fas Receptor - immunology; fas Receptor - pharmacology; Fas-associated phosphatase-1; Hematologic and hematopoietic diseases; Human T-cell leukemia virus type-I; human T-lymphotropic virus 1; Human T-lymphotropic virus 1 - genetics; Human T-lymphotropic virus 1 - isolation & purification; Humans; Leukemia, T-Cell - genetics; Leukemia, T-Cell - metabolism; Leukemia, T-Cell - pathology; Leukemia, T-Cell - virology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Protein Phosphatase 1; Protein Tyrosine Phosphatase, Non-Receptor Type 13; Protein Tyrosine Phosphatases - biosynthesis; Protein Tyrosine Phosphatases - genetics; Protein Tyrosine Phosphatases - immunology; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; Tumor Cells, Cultured; Virus Integration - genetics
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(99)00313-4

A preventive role for human T-cell leukemia virus type-I (HTLV-I) and Fas-associated phosphatase-1 (FAP-1) in Fas-mediated apoptosis has been reported in HTLV-I-infected cells. In the present study, we examined whether these molecules increased during the acquisition of Fas-resistance in adult T-cell leukemia (ATL) cell lines. SO4, ST1 and KK1 are Fas-sensitive ATL cell lines, and produce small amounts of HTLV-I in vitro. Although their subclones RSO4 and RST1 are completely Fas-resistant, they produced an equivalent amount of HTLV-I to SO4 and ST1. Moreover, FAP-1 mRNA was not detected in these cell lines irrespective of Fas sensitivity. Thus, Fas resistance in ATL cells was not directly associated with the increased production of HTLV-I or FAP-1.