Histone Deacetylase Inhibitor Activates the p21/WAF1/Cip1 Gene Promoter through the Sp1 Sites

Trichostatin A (TSA), a specific histone deacetylase inhibitor, induces histone hyperacetylation and modulates the expression of some genes. We examined the effects of TSA on MG63 cells. TSA induced growth arrest and expression of the p21/WAF1/Cip1 protein. A close correlation between the level of h...

Full description

Saved in:
Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1999-12, Vol.886 (1), p.195-199
Main Authors: SOWA, Y., ORITA, T., HIRANABE-MINAMIKAWA, S., NAKANO, K., MIZUNO, T., NOMURA, H., SAKAI, T.
Format: Article
Language:English
Subjects:
Cyclin-Dependent Kinase Inhibitor p21
Cyclins - genetics
Enzyme Inhibitors - pharmacology
Histone Deacetylase Inhibitors
Humans
Hydroxamic Acids - pharmacology
Promoter Regions, Genetic
Sp1 Transcription Factor - metabolism
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Trichostatin A (TSA), a specific histone deacetylase inhibitor, induces histone hyperacetylation and modulates the expression of some genes. We examined the effects of TSA on MG63 cells. TSA induced growth arrest and expression of the p21/WAF1/Cip1 protein. A close correlation between the level of histone acetylation and induction of the p21/WAF1/Cip1 protein was detected. Using several mutant p21/WAF1/Cip1 promoter fragments, mutation of either of two Sp1 sites at -82 or -69 of the p21/WAF1/Cip1 promoter reduced the responsiveness to TSA. This finding indicates that TSA activates the p21/WAF1/Cip1 promoter through the Sp1 sites in a p53-independent manner.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1999.tb09415.x